Pumping-induced perturbation of flow in microfluidic channels and its implications for on-chip cell culture

Jianhua Zhou, Kangning REN, Wen Dai, Yihua Zhao, Declan Ryan, Hongkai Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

We study the rate of response to changes in the rate of flow and the perturbations in flow in polydimethylsiloxane (PDMS) microfluidic chips that are subjected to several common flow-control systems. We find that the flow rate of liquid delivered from a syringe pump equipped with a glass syringe responds faster to the changes in the conditions of flow than the same liquid delivered from a plastic syringe; and the rate of flow delivered from compressed air responds faster than that from a glass syringe. We discover that the rate of flow that is driven by a syringe pump and regulated by an integrated pneumatic valve responds even faster, but this flow-control method is characterized by large perturbations. We also examine the possible effects of these large perturbations on NIH 3T3 cells in microfluidic channels and find that they could cause the detachment of NIH 3T3 cells in the microchannels.

Original languageEnglish
Pages (from-to)2288-2294
Number of pages7
JournalLab on a Chip
Volume11
Issue number13
DOIs
Publication statusPublished - 7 Jul 2011

Scopus Subject Areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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