Pseudolaric acid B suppresses T lymphocyte activation through inhibition of NF-κB signaling pathway and p38 phosphorylation

Ting Li, Vincent Kam Wai Wong, Qin Yi Xiao, Fan Wong Yuen, Hua Zhou*, Liang LIU

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Pseudolaric acid B (PAB) is a major bioactive component of the medicinal plant Pseudolarix kaempferi. Traditional medicine practitioners in Asia have been using the roots of this plant to treat inflammatory and microbial skin diseases for centuries. In the current study, in vitro immunosuppressive effect of PAB and the underlying mechanisms have been investigated. The results showed that PAB dose-dependently suppressed human T lymphocyte proliferation, IL-2 production and CD25 expression induced by co-stimulation of PMA plus ionomycin or of anti-OKT-3 plus anti-CD28. Mechanistic studies showed that PAB significantly inhibited nuclear translocation of NF-κB p65 and phosphorylation and degradation of IκB-α evoked by co-stimulation of PMA plus ionomycin. PAB could also suppress the phosphorylation of p38 in the MAPKs pathway. Based on these evidences, we conclude that PAB suppressed T lymphocyte activation through inhibition of NFκB and p38 signaling pathways; this would make PAB a strong candidate for further study as an anti-inflammatory agent.

Original languageEnglish
Pages (from-to)87-95
Number of pages9
JournalJournal of Cellular Biochemistry
Volume108
Issue number1
DOIs
Publication statusPublished - 1 Sep 2009

Scopus Subject Areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

User-Defined Keywords

  • CD25
  • NF-κB
  • p38
  • Pseudolaric acid B
  • T lymphocyte activation

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