Protocol for metabolic synergy analysis of multi-organs in mice using iMS2Net

Jiyang Dong, Qianwen Peng, Lingli Deng, Chao Zhao*, Zongwei Cai*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review


Biomolecule regulation and communication between cells and organs show specific network and regional features and play an important role in disease progression. Here, we describe steps for in situ detection of biomolecules with detailed spatial distribution using imaging mass spectrometry (iMS). Using the information on inter-cells and inter-organs metabolic interactions provided by iMS, we detail the establishment of an iMS-dataset-sourced multiscale network strategy and present steps for exploring metabolic responses in environmental-exposure-induced disease model. For complete details on the use and execution of this protocol, please refer to Dong et al. (2022).1

Original languageEnglish
Article number102159
Number of pages16
JournalSTAR Protocols
Issue number2
Early online date13 Mar 2023
Publication statusPublished - 16 Jun 2023

Scopus Subject Areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

User-Defined Keywords

  • Bioinformatics
  • Biotechnology and bioengineering
  • Computer sciences
  • Environmental sciences
  • Metabolism


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