TY - JOUR
T1 - Proteomics investigation on aristolochic acid nephropathy
T2 - A case study on rat kidney tissues
AU - Wu, Han Zhi
AU - Guo, Lin
AU - Mak, Yuen Fun
AU - Liu, Ning
AU - Poon, Wing Tat
AU - Chan, Yan Wo
AU - CAI, Zongwei
N1 - Funding Information:
Acknowledgements Financial support from the Food and Health Bureau and the Health and Health Services Research Fund of Hong Kong (05060141) is acknowledged.
PY - 2011/4
Y1 - 2011/4
N2 - Prolonged intake of aristolochic acid (AA) has been shown to be associated with the development of certain renal disorders. Renal tubular atrophy and interstitial fibrosis are the early symptoms of AA nephropathy. The symptoms were observed in rats that were dosed with AA at a dosage of 10 mg/kg/day for 1 month. Apart from the renal tubular atrophy and interstitial fibrosis, AA-DNA adducts were detected in the rat kidney tissue. Differentiated proteins were identified in the kidney tissues from proteomics investigations. The upregulated proteins identified included ornithine aminotransferase, sorbitol dehydrogenase, actin, aspartoacylase, 3-hydroxyisobutyrate dehydrogenase, and peroxiredoxin-1. Downregulated proteins such as ATP synthase subunit β, glutamate dehydrogenase 1, regucalcin, glutamate-cysteine ligase regulatory subunit, dihydropteridine reductase, hydroxyacyl-coenzyme A dehydrogenase, voltage-dependent anion-selective channel protein 1, prohibitin, and adenylate kinase isoenzyme 4 were also identified. Several identified protein markers were found to have biological and medical significance.
AB - Prolonged intake of aristolochic acid (AA) has been shown to be associated with the development of certain renal disorders. Renal tubular atrophy and interstitial fibrosis are the early symptoms of AA nephropathy. The symptoms were observed in rats that were dosed with AA at a dosage of 10 mg/kg/day for 1 month. Apart from the renal tubular atrophy and interstitial fibrosis, AA-DNA adducts were detected in the rat kidney tissue. Differentiated proteins were identified in the kidney tissues from proteomics investigations. The upregulated proteins identified included ornithine aminotransferase, sorbitol dehydrogenase, actin, aspartoacylase, 3-hydroxyisobutyrate dehydrogenase, and peroxiredoxin-1. Downregulated proteins such as ATP synthase subunit β, glutamate dehydrogenase 1, regucalcin, glutamate-cysteine ligase regulatory subunit, dihydropteridine reductase, hydroxyacyl-coenzyme A dehydrogenase, voltage-dependent anion-selective channel protein 1, prohibitin, and adenylate kinase isoenzyme 4 were also identified. Several identified protein markers were found to have biological and medical significance.
KW - Aristolochic acid
KW - Kidney disease
KW - Protein biomarker
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=79954451425&partnerID=8YFLogxK
U2 - 10.1007/s00216-010-4463-4
DO - 10.1007/s00216-010-4463-4
M3 - Journal article
C2 - 21152904
AN - SCOPUS:79954451425
SN - 1618-2642
VL - 399
SP - 3431
EP - 3439
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 10
ER -