Proteomic characterization of Phragmites communis in ecotypes of swamp and desert dune

Suxia Cui, Jia Hu, Bin Yang, Lu Shi, Fang Huang, Sau Na Tsai, Sai Ming Ngai, Yikun He, Jianhua ZHANG*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

23 Citations (Scopus)


Phragmites communis Trin. (common reed) is a recognized model plant for studying its adaptation to contrasting and harsh environments. To understand the inherent molecular basis for its remarkable resistance to combined stresses, we performed a comprehensive proteomic analysis of the leaf proteins from two ecotypes, i.e. swamp and desert dune, naturally growing in the desert region of northwestern China. First, a proteome reference map of Phragmites was established based on the swamp ecotype. Proteins were resolved by 2-D/SDS-PAGE and identified by MALDI-TOF/TOF MS. In total, 177 spots were identified corresponding to 51 proteins. The major proteins identified are proteins involved in photosynthesis, glutathione and ascorbic acid metabolism as well as protein synthesis and quality control. Second, the 2-DE profiles of the two ecotypes were compared quantitatively via DIGE analysis. Compared with swamp ecotype, 51 proteins spots are higher-expressed and 58 protein spots are lower-expressed by twofold or more in desert dune ecotype. Major differences were found for the proteins involved in light reaction of photosynthesis, protein biosynthesis and quality control and antioxidative reactions. The physiological significance of such differences is discussed in the context of a flow of complex events in relation to plant adaptation to combined environmental stresses.

Original languageEnglish
Pages (from-to)3950-3967
Number of pages18
Issue number16
Publication statusPublished - Aug 2009

Scopus Subject Areas

  • Biochemistry
  • Molecular Biology

User-Defined Keywords

  • 2-D DIGE
  • Adaptation
  • Ecotype
  • Phragmites communis
  • Plant proteomics


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