TY - JOUR
T1 - Proteomic Basis of Stress Responses in the Gills of the Pacific Oyster Crassostrea gigas
AU - Zhang, Yang
AU - Sun, Jin
AU - Mu, Huawei
AU - Li, Jun
AU - Zhang, Yuehuan
AU - Xu, Fengjiao
AU - Xiang, Zhiming
AU - Qian, Pei Yuan
AU - Qiu, Jian Wen
AU - Yu, Ziniu
N1 - Funding information:
This study was supported by the Joint Funds of NSFC-Guangdong (no. U1201215), the National Basic Research Program of China (no. 2010CB126404), and University Grant Committee of Hong Kong (no. GRF 261312). The mass spectrometry was performed in Instrumental Analysis & Research Center, Sun Yat-Sen University. We thank Miss Ling Fang for assistance with the mass spectrometry.
Publisher copyright:
© 2014 American Chemical Society
PY - 2015/1/2
Y1 - 2015/1/2
N2 - The Pacific oyster Crassostrea gigas is one of the dominant sessile inhabitants of the estuarine intertidal zone, which is a physically harsh environment due to the presence of a number of stressors. Oysters have adapted to highly dynamic and stressful environments, but the molecular mechanisms underlying such stress adaptation are largely unknown. In the present study, we examined the proteomic responses in the gills of C. gigas exposed to three stressors (high temperature, low salinity, and aerial exposure) they often encounter in the field. We quantitatively compared the gill proteome profiles using iTRAQ-coupled 2-D LC-MS/MS. There were 3165 identified proteins among which 2379 proteins could be quantified. Heat shock, hyposalinity, and aerial exposure resulted in 50, 15, and 33 differentially expressed gill proteins, respectively. Venn diagram analysis revealed substantial different responses to the three stressors. Only xanthine dehydrogenase/oxidase showed a similar expression pattern across the three stress treatments, suggesting that reduction of ROS accumulation may be a conserved response to these stressors. Heat shock caused significant overexpression of molecular chaperones and production of S-adenosyl-l-methionine, indicating their crucial protective roles against protein denature. In addition, heat shock also activated immune responses, Ca2+ binding protein expression. By contrast, hyposalinity and aerial exposure resulted in the up-regulation of 3-demethylubiquinone-9 3-methyltransferase, indicating that increase in ubiquinone synthesis may contribute to withstanding both the osmotic and desiccation stress. Strikingly, the majority of desiccation-responsive proteins, including those involved in metabolism, ion transportation, immune responses, DNA duplication, and protein synthesis, were down-regulated, indicating conservation of energy as an important strategy to cope with desiccation stress. There was a high consistency between the expression levels determined by iTRAQ and Western blotting, highlighting the high reproducibility of our proteomic approach and its great value in revealing molecular mechanisms of stress responses.
AB - The Pacific oyster Crassostrea gigas is one of the dominant sessile inhabitants of the estuarine intertidal zone, which is a physically harsh environment due to the presence of a number of stressors. Oysters have adapted to highly dynamic and stressful environments, but the molecular mechanisms underlying such stress adaptation are largely unknown. In the present study, we examined the proteomic responses in the gills of C. gigas exposed to three stressors (high temperature, low salinity, and aerial exposure) they often encounter in the field. We quantitatively compared the gill proteome profiles using iTRAQ-coupled 2-D LC-MS/MS. There were 3165 identified proteins among which 2379 proteins could be quantified. Heat shock, hyposalinity, and aerial exposure resulted in 50, 15, and 33 differentially expressed gill proteins, respectively. Venn diagram analysis revealed substantial different responses to the three stressors. Only xanthine dehydrogenase/oxidase showed a similar expression pattern across the three stress treatments, suggesting that reduction of ROS accumulation may be a conserved response to these stressors. Heat shock caused significant overexpression of molecular chaperones and production of S-adenosyl-l-methionine, indicating their crucial protective roles against protein denature. In addition, heat shock also activated immune responses, Ca2+ binding protein expression. By contrast, hyposalinity and aerial exposure resulted in the up-regulation of 3-demethylubiquinone-9 3-methyltransferase, indicating that increase in ubiquinone synthesis may contribute to withstanding both the osmotic and desiccation stress. Strikingly, the majority of desiccation-responsive proteins, including those involved in metabolism, ion transportation, immune responses, DNA duplication, and protein synthesis, were down-regulated, indicating conservation of energy as an important strategy to cope with desiccation stress. There was a high consistency between the expression levels determined by iTRAQ and Western blotting, highlighting the high reproducibility of our proteomic approach and its great value in revealing molecular mechanisms of stress responses.
KW - aerial exposure
KW - Crassostrea
KW - gills
KW - iTRAQ
KW - oyster
KW - salinity
KW - temperature
UR - http://www.scopus.com/inward/record.url?scp=84920279292&partnerID=8YFLogxK
U2 - 10.1021/pr500940s
DO - 10.1021/pr500940s
M3 - Journal article
C2 - 25389644
AN - SCOPUS:84920279292
SN - 1535-3893
VL - 14
SP - 304
EP - 317
JO - Journal of Proteome Research
JF - Journal of Proteome Research
IS - 1
ER -