Proteomic analysis of secreted proteins by human bronchial epithelial cells in response to cadmium toxicity

De Ju Chen, Yan Ming Xu, Wei Zheng, Dong Yang Huang, Wing Yan Wong, William C S TAI, Yong Yeon Cho, Andy T.Y. Lau*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

For years, many studies have been conducted to investigate the intracellular response of cells challenged with toxic metal(s), yet, the corresponding secretome responses, especially in human lung cells, are largely unexplored. Here, we provide a secretome analysis of human bronchial epithelial cells (BEAS-2B) treated with cadmium chloride (CdCl2), with the aim of identifying secreted proteins in response to Cd toxicity. Proteins from control and spent media were separated by two-dimensional electrophoresis and visualized by silver staining. Differentially-secreted proteins were identified by MALDI-TOF-MS analysis and database searching. We characterized, for the first time, the extracellular proteome changes of BEAS-2B dosed with Cd. Our results unveiled that Cd treatment led to the marked upregulation of molecular chaperones, antioxidant enzymes, enzymes associated with glutathione metabolic process, proteins involved in cellular energy metabolism, as well as tumor-suppressors. Pretreatment of cells with the thiol antioxidant glutathione before Cd treatment effectively abrogated the secretion of these proteins and prevented cell death. Taken together, our results demonstrate that Cd causes oxidative stress-induced cytotoxicity; and the differentially-secreted protein signatures could be considered as targets for potential use as extracellular biomarkers upon Cd exposure.

Original languageEnglish
Pages (from-to)3075-3086
Number of pages12
JournalProteomics
Volume15
Issue number17
DOIs
Publication statusPublished - 1 Sep 2015

Scopus Subject Areas

  • Biochemistry
  • Molecular Biology

User-Defined Keywords

  • BEAS-2B
  • Biomedicine
  • Cadmium
  • Human lung cells
  • Mass spectrometry
  • Secretomics

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