Proteomic analysis of protective effects of polysaccharides from Salvia miltiorrhiza against immunological liver injury in mice

Xue Gang Sun, Xiu Qiong Fu, Hong Bing Cai, Qiang Liu, Chun Hua Li, Ya Wei Liu, Ying Jia Li, Zhi Feng Liu, Yu Hong Song, Zhi Ping Lv*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

21 Citations (Scopus)

Abstract

This study was designed to investigate mechanisms of the protective effects of Salvia miltiorrhiza polysaccharide (SMPS) against lipopolysaccharide (LPS)-induced immunological liver injury (ILI) in Bacille Calmette-Guérin (BCG)-primed mice. Two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis showed that three proteins are down-regulated and six proteins are up-regulated by SMPS. SMPS reduces the degree of liver injury by up-regulating the enzymes of the citric acid cycle, namely malate dehydrogenase (MDH) and 2-oxoglutarate dehydrogenase complex. LPS significantly increases nuclear factor kappa B (NF-κB) activation, inducible nitric oxide synthase (iNOS) expression and MDA level in BCG primed mice liver, whereas SMPS treatment protects against the immunological liver injury through inhibitionof the NF-κB activation by up-regulation of PRDX6 and the subsequent attenuation of lipid peroxidation, iNOS expression and inflammation.

Original languageEnglish
Pages (from-to)1087-1094
Number of pages8
JournalPhytotherapy Research
Volume25
Issue number7
Early online date8 Apr 2011
DOIs
Publication statusPublished - Jul 2011

Scopus Subject Areas

  • Pharmacology

User-Defined Keywords

  • Immunological liver injury
  • Polysaccharides
  • PRDX6
  • Proteomics
  • Salvia miltiorrhiza

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