Proteomic analysis of chromium cytotoxicity in cultured rat lung epithelial cells

Ting Lei, Qing Yu He*, Zhen Cai, Yuan Zhou, Yi Li Wang, Lu Sheng Si, Zongwei CAI, Jen Fu Chiu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Chromium (Cr) has been widely used in industry for more than one century. Exposure to hexavalent Cr compounds is strongly associated with increasing risk of lung cancer. Extensive researches at DNA level indicated that generation of ROS from the reduction of Cr(VI) leading to DNA damage is the major cause of the toxicity and carcinogenicity of Cr(VI). The present study in cellular and protein levels confirmed that Cr(VI) induced apoptosis of lung epithelial cells (LEC) via ROS generation. To view the differentially expressed proteins in the process of Cr(VI) reduction, subcellular proteomics was applied and allowed the identification of more than 30 proteins with expression alteration. Most of those proteins are correlated with ROS-elicited responses, which were further validated by Western blotting analysis, induction of p53 pathway and antioxidative treatment. The current findings provided additional evidence in protein level to support the claim that ROS generated during the process of Cr(VI) reduction are involved in the Cr(VI)-induced toxicity and carcinogenesis.

Original languageEnglish
Pages (from-to)2420-2429
Number of pages10
JournalProteomics
Volume8
Issue number12
DOIs
Publication statusPublished - Jun 2008

Scopus Subject Areas

  • Biochemistry
  • Molecular Biology

User-Defined Keywords

  • Chromium
  • Cytotoxicity
  • Reactive oxygen species
  • Subcellular proteomics

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