TY - JOUR
T1 - Protein-Metal Organic Framework Hybrid Composites with Intrinsic Peroxidase-like Activity as a Colorimetric Biosensing Platform
AU - Yin, Yuqing
AU - Gao, Chenling
AU - Xiao, Qi
AU - Lin, Guo
AU - Lin, Zian
AU - Cai, Zongwei
AU - Yang, Huanghao
N1 - Funding Information:
This work was financially supported by the National Natural Science Foundation of China (21375018 and 21675025), the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT15R11), and the Natural Science Foundation of Fujian Province (2014J01402).
Publisher copyright:
© 2016 American Chemical Society
PY - 2016/10/26
Y1 - 2016/10/26
N2 - Artificial enzyme mimetics have received considerable attention because natural enzymes have some significant drawbacks, including enzyme autolysis, low catalytic activity, poor recovery, and low stability to environmental changes. Herein, we demonstrated a facile approach for one-pot synthesis of hemeprotein-metal organic framework hybrid composites (H-MOFs) by using bovine hemoglobin (BHb) and zeolitic imidazolate framework-8 (ZIF-8) as a model reaction system. Surprisingly, the new hybrid composites exhibit 423% increase in peroxidase-like catalytic activity compared to free BHb. Taking advantages of the unique pore structure of H-MOFs with high catalytic property, a H-MOFs-based colorimetric biosensing platform was newly constructed and applied for the fast and sensitive detection of hydrogen peroxide (H2O2) and phenol. The corresponding detection limits as low as 1.0 μM for each analyte with wide linear ranges (0-800 μM for H2O2 and 0-200 μM for phenol) were obtained by naked-eye visualization. Significantly, a sensitive and selective method for visual assay of trace H2O2 in cells and phenol in sewage was achieved with this platform. The stability of H-MOFs was also examined, and excellent reproducibility and recyclability without losing in their activity were observed. In addition, the general applicability of H-MOFs was also investigated by using other hemeproteins (horseradish peroxidase, and myoglobin), and the corresponding catalytic activities were 291% and 273% enhancement, respectively. This present work not only expands the application of MOFs but also provides an alternative technique for biological and environmental sample assay.
AB - Artificial enzyme mimetics have received considerable attention because natural enzymes have some significant drawbacks, including enzyme autolysis, low catalytic activity, poor recovery, and low stability to environmental changes. Herein, we demonstrated a facile approach for one-pot synthesis of hemeprotein-metal organic framework hybrid composites (H-MOFs) by using bovine hemoglobin (BHb) and zeolitic imidazolate framework-8 (ZIF-8) as a model reaction system. Surprisingly, the new hybrid composites exhibit 423% increase in peroxidase-like catalytic activity compared to free BHb. Taking advantages of the unique pore structure of H-MOFs with high catalytic property, a H-MOFs-based colorimetric biosensing platform was newly constructed and applied for the fast and sensitive detection of hydrogen peroxide (H2O2) and phenol. The corresponding detection limits as low as 1.0 μM for each analyte with wide linear ranges (0-800 μM for H2O2 and 0-200 μM for phenol) were obtained by naked-eye visualization. Significantly, a sensitive and selective method for visual assay of trace H2O2 in cells and phenol in sewage was achieved with this platform. The stability of H-MOFs was also examined, and excellent reproducibility and recyclability without losing in their activity were observed. In addition, the general applicability of H-MOFs was also investigated by using other hemeproteins (horseradish peroxidase, and myoglobin), and the corresponding catalytic activities were 291% and 273% enhancement, respectively. This present work not only expands the application of MOFs but also provides an alternative technique for biological and environmental sample assay.
KW - colorimetric detection
KW - hemeproteins
KW - hydrogen peroxide
KW - metal-organic framework
KW - peroxidase mimetics
KW - phenol
UR - http://www.scopus.com/inward/record.url?scp=84993945011&partnerID=8YFLogxK
U2 - 10.1021/acsami.6b09893
DO - 10.1021/acsami.6b09893
M3 - Journal article
C2 - 27700042
AN - SCOPUS:84993945011
SN - 1944-8244
VL - 8
SP - 29052
EP - 29061
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 42
ER -