Protein from red cabbage (Brassica oleracea) seeds with antifungal, antibacterial, and anticancer activities

Xiu Juan Ye; Tzi Bun Ng; Zu Jian Wu; Lian Hui  Xie; Evandro Fei Fang; Jack Ho Wong; Wen Liang Pan; Stephen Cho Wing Sze; Yan Bo Zhang

doi:10.1021/jf201874j

Protein from red cabbage (Brassica oleracea) seeds with antifungal, antibacterial, and anticancer activities

Xiu Juan Ye^*, Tzi Bun Ng^*, Zu Jian Wu, Lian Hui Xie, Evandro Fei Fang, Jack Ho Wong, Wen Liang Pan, Stephen Cho Wing Sze, Yan Bo Zhang^*

^*Corresponding author for this work

Golden Meditech Centre for NeuroRegeneration Sciences

Research output: Contribution to journal › Journal article › peer-review

19 Citations (Scopus)

Abstract

A 30 kDa antifungal protein was purified from red cabbage (Brassica oleracea) seeds. It exhibited a molecular mass and N-terminal amino acid sequence disinct from those of previously isolated Brassica antifungal proteins. The protocol used entailed ion exchange chromatography on Q-Sepharose and SP-Sepharose followed by fast protein liquid chromatography on Mono S. The protein hindered mycelial growth in Mycosphaerella arachidicola (with an IC₅₀ = 5 μM), Setospaeria turcica, and Bipolaris maydis. It also inhibited the yeast Candida albicans with an IC₅₀ = 96 μM. It exerted its antifungal action by permeabilizing the fungal membrane as evidenced by staining with Sytox green. The antifungal activity was stable from pH 3 to 11 and from 0 to 65 °C. It manifested antibacterial activity against Pseudomonas aeruginosa (IC50 = 53 μM). Furthermore, after 48 h of culture, it suppressed proliferation of nasopharyngeal cancer and hepatoma cells with IC50 = 50 and 90 μM, respectively.

Original language	English
Pages (from-to)	10232–10238
Number of pages	7
Journal	Journal of Agricultural and Food Chemistry
Volume	59
Issue number	18
DOIs	https://doi.org/10.1021/jf201874j
Publication status	Published - 28 Sept 2011

Scopus Subject Areas

General Medicine

User-Defined Keywords

Brassica oleracea
seeds
purification
antifungal protein

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10.1021/jf201874j

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title = "Protein from red cabbage (Brassica oleracea) seeds with antifungal, antibacterial, and anticancer activities",

abstract = "A 30 kDa antifungal protein was purified from red cabbage (Brassica oleracea) seeds. It exhibited a molecular mass and N-terminal amino acid sequence disinct from those of previously isolated Brassica antifungal proteins. The protocol used entailed ion exchange chromatography on Q-Sepharose and SP-Sepharose followed by fast protein liquid chromatography on Mono S. The protein hindered mycelial growth in Mycosphaerella arachidicola (with an IC50 = 5 μM), Setospaeria turcica, and Bipolaris maydis. It also inhibited the yeast Candida albicans with an IC50 = 96 μM. It exerted its antifungal action by permeabilizing the fungal membrane as evidenced by staining with Sytox green. The antifungal activity was stable from pH 3 to 11 and from 0 to 65 °C. It manifested antibacterial activity against Pseudomonas aeruginosa (IC50 = 53 μM). Furthermore, after 48 h of culture, it suppressed proliferation of nasopharyngeal cancer and hepatoma cells with IC50 = 50 and 90 μM, respectively.",

keywords = "Brassica oleracea, seeds, purification, antifungal protein",

author = "Ye, {Xiu Juan} and Ng, {Tzi Bun} and Wu, {Zu Jian} and Xie, {Lian Hui} and Fang, {Evandro Fei} and Wong, {Jack Ho} and Pan, {Wen Liang} and Sze, {Stephen Cho Wing} and Zhang, {Yan Bo}",

note = "Funding information: The award of a grant (Research Fund for the Control of Infections Diseases, Grant 09080432) from the Food and Health Bureau, Government of Hong Kong Special Administrative Region, is gratefully acknowledged. Publisher copyright: {\textcopyright} 2011 American Chemical Society",

year = "2011",

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doi = "10.1021/jf201874j",

language = "English",

volume = "59",

pages = "10232–10238",

journal = "Journal of Agricultural and Food Chemistry",

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T1 - Protein from red cabbage (Brassica oleracea) seeds with antifungal, antibacterial, and anticancer activities

AU - Ye, Xiu Juan

AU - Ng, Tzi Bun

AU - Wu, Zu Jian

AU - Xie, Lian Hui

AU - Fang, Evandro Fei

AU - Wong, Jack Ho

AU - Pan, Wen Liang

AU - Sze, Stephen Cho Wing

AU - Zhang, Yan Bo

N1 - Funding information: The award of a grant (Research Fund for the Control of Infections Diseases, Grant 09080432) from the Food and Health Bureau, Government of Hong Kong Special Administrative Region, is gratefully acknowledged. Publisher copyright: © 2011 American Chemical Society

PY - 2011/9/28

Y1 - 2011/9/28

N2 - A 30 kDa antifungal protein was purified from red cabbage (Brassica oleracea) seeds. It exhibited a molecular mass and N-terminal amino acid sequence disinct from those of previously isolated Brassica antifungal proteins. The protocol used entailed ion exchange chromatography on Q-Sepharose and SP-Sepharose followed by fast protein liquid chromatography on Mono S. The protein hindered mycelial growth in Mycosphaerella arachidicola (with an IC50 = 5 μM), Setospaeria turcica, and Bipolaris maydis. It also inhibited the yeast Candida albicans with an IC50 = 96 μM. It exerted its antifungal action by permeabilizing the fungal membrane as evidenced by staining with Sytox green. The antifungal activity was stable from pH 3 to 11 and from 0 to 65 °C. It manifested antibacterial activity against Pseudomonas aeruginosa (IC50 = 53 μM). Furthermore, after 48 h of culture, it suppressed proliferation of nasopharyngeal cancer and hepatoma cells with IC50 = 50 and 90 μM, respectively.

AB - A 30 kDa antifungal protein was purified from red cabbage (Brassica oleracea) seeds. It exhibited a molecular mass and N-terminal amino acid sequence disinct from those of previously isolated Brassica antifungal proteins. The protocol used entailed ion exchange chromatography on Q-Sepharose and SP-Sepharose followed by fast protein liquid chromatography on Mono S. The protein hindered mycelial growth in Mycosphaerella arachidicola (with an IC50 = 5 μM), Setospaeria turcica, and Bipolaris maydis. It also inhibited the yeast Candida albicans with an IC50 = 96 μM. It exerted its antifungal action by permeabilizing the fungal membrane as evidenced by staining with Sytox green. The antifungal activity was stable from pH 3 to 11 and from 0 to 65 °C. It manifested antibacterial activity against Pseudomonas aeruginosa (IC50 = 53 μM). Furthermore, after 48 h of culture, it suppressed proliferation of nasopharyngeal cancer and hepatoma cells with IC50 = 50 and 90 μM, respectively.

KW - Brassica oleracea

KW - seeds

KW - purification

KW - antifungal protein

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U2 - 10.1021/jf201874j

DO - 10.1021/jf201874j

M3 - Journal article

SN - 0021-8561

VL - 59

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JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 18

ER -

Protein from red cabbage (Brassica oleracea) seeds with antifungal, antibacterial, and anticancer activities

Abstract

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