Protein from red cabbage (Brassica oleracea) seeds with antifungal, antibacterial, and anticancer activities

Xiu Juan Ye*, Tzi Bun Ng*, Zu Jian Wu, Lian Hui Xie, Evandro Fei Fang, Jack Ho Wong, Wen Liang Pan, Stephen Cho Wing Sze, Yan Bo Zhang*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

17 Citations (Scopus)


A 30 kDa antifungal protein was purified from red cabbage (Brassica oleracea) seeds. It exhibited a molecular mass and N-terminal amino acid sequence disinct from those of previously isolated Brassica antifungal proteins. The protocol used entailed ion exchange chromatography on Q-Sepharose and SP-Sepharose followed by fast protein liquid chromatography on Mono S. The protein hindered mycelial growth in Mycosphaerella arachidicola (with an IC50 = 5 μM), Setospaeria turcica, and Bipolaris maydis. It also inhibited the yeast Candida albicans with an IC50 = 96 μM. It exerted its antifungal action by permeabilizing the fungal membrane as evidenced by staining with Sytox green. The antifungal activity was stable from pH 3 to 11 and from 0 to 65 °C. It manifested antibacterial activity against Pseudomonas aeruginosa (IC50 = 53 μM). Furthermore, after 48 h of culture, it suppressed proliferation of nasopharyngeal cancer and hepatoma cells with IC50 = 50 and 90 μM, respectively.
Original languageEnglish
Pages (from-to)10232–10238
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Issue number18
Publication statusPublished - 28 Sept 2011

Scopus Subject Areas

  • Medicine(all)

User-Defined Keywords

  • Brassica oleracea
  • seeds
  • purification
  • antifungal protein


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