Protective effects of dibenzocyclooctadiene lignans from Schisandra chinensis against beta-amyloid and homocysteine neurotoxicity in PC12 cells

Juxian SONG, Xiang Lin, Ricky N S WONG, Cho Wing SZE, Yao Tong, Pang Chui Shaw, Yan Bo Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)

Abstract

Aggregated beta-amyloid (Aβ) and elevated plasma levels of homocysteine have been implicated as critical factors in the pathogenesis of Alzheimer's disease. The neuroprotective effects and possible mechanism of four structurally similar dibenzocyclooctadiene lignans (namely schisandrin, schisantherin A, schisandrin B and schisandrin C) isolated from the fruit of Schisandra chinensis (Turcz.) Baill. (Schisandraceae) againstAβ 25-35 and homocysteine toxicity in PC12 cells was studied. Exposure of PC12 cells to 0.5 μM Aβ25-35 caused significant cell death, increased the number of apoptotic cells, elevated reactive oxygen species, increased the levels of the pro-apoptotic protein Bax and caspase-3 activation. All these effects induced byAβ25-35 were markedly reversed by schisandrin B and schisandrin C pretreatment, while schisandrin and schisantherin A had no obvious effects. Meanwhile, schisandrin B and schisandrin C reversed homocysteine-induced cytotoxicity. The results indicated that schisandrin B and schisandrin C protected PC12 cells against Aβ toxicity by attenuating ROS production and modulating the apoptotic signal pathway through Bax and caspase-3. Further structure-activity analysis of Schisandra lignans and evaluations of their neuroprotective effects using AD animal models are warranted.

Original languageEnglish
Pages (from-to)435-443
Number of pages9
JournalPhytotherapy Research
Volume25
Issue number3
DOIs
Publication statusPublished - Mar 2011

Scopus Subject Areas

  • Pharmacology

User-Defined Keywords

  • Beta-amyloid
  • Dibenzocyclooctadiene lignans
  • Homocysteine
  • Schisandra chinensis (Turcz.) Baill.
  • Schisandrin B
  • Schisandrin C

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