TY - JOUR
T1 - Promotion of bone repair by implantation of cryopreserved bone marrowderived mononuclear cells in a rabbit model of steroid-associated osteonecrosis
AU - Xie, Xin Hui
AU - Wang, Xin Luan
AU - He, Yi Xin
AU - Liu, Zhong
AU - Sheng, Hui
AU - Zhang, Ge
AU - Qin, Ling
N1 - Hong Kong General Research Fund. Grant Numbers: GRF CUHK 4725/08, GRF CUHK 4737/10
Publisher Copyright:
© 2012 by the American College of Rheumatology
PY - 2012/5
Y1 - 2012/5
N2 - Objective. Cytotherapy is an insufficient method for promoting bone repair in steroid-associated osteonecrosis (SAON), and this has been attributed to impairment of the bioactivity of bone marrowderived stem cells (BMSCs) after pulsed administration of steroids. Cryopreserved autologous bone marrow derived mononuclear cells (BMMNCs), which contain BMSCs, might maintain their bioactivity in vitro. This study sought to investigate the effects of cryopreserved BMMNCs, before steroid administration, on the enhancement of bone repair in an established rabbit model of SAON. Methods. For in vitro study, bone marrow was harvested 4 weeks before SAON induction from the iliac crests of rabbits (n = 10) to isolate fresh BMMNCs, and the BMMNCs were then cryopreserved for 8 weeks. Both the fresh and the cryopreserved BMMNCs were evaluated for their bioactivity and osteogenic differentiation capacity. In addition, BMMNCs were isolated 2 weeks after SAON induction and subjected to the same evaluations. For in vivo study, cryopreserved BMMNCs were implanted into the bone tunnel during core decompression of the femur (n = 12 rabbits) after the induction of SAON, and tissue regeneration was evaluated by microcomputed tomography and histologic analyses at 12 weeks postoperation. Results. In vitro, there were no significant differences in the bioactivity or ability to undergo osteogenic differentiation between fresh BMMNCs and cryopreserved BMMNCs, but after SAON induction, both features were decreased significantly. In vivo, the bone mineral density, ratio of bone volume to total volume of bone, and volume and diameter of neovascularization within the bone tunnel were significantly higher in the BMMNC-treated group compared to the nontreated control group at 12 weeks postoperation. Conclusion. Cryopreserved BMMNCs maintained their bioactivity and promoted bone regeneration and neovascularization within the bone tunnel after core decompression in this rabbit model of SAON.
AB - Objective. Cytotherapy is an insufficient method for promoting bone repair in steroid-associated osteonecrosis (SAON), and this has been attributed to impairment of the bioactivity of bone marrowderived stem cells (BMSCs) after pulsed administration of steroids. Cryopreserved autologous bone marrow derived mononuclear cells (BMMNCs), which contain BMSCs, might maintain their bioactivity in vitro. This study sought to investigate the effects of cryopreserved BMMNCs, before steroid administration, on the enhancement of bone repair in an established rabbit model of SAON. Methods. For in vitro study, bone marrow was harvested 4 weeks before SAON induction from the iliac crests of rabbits (n = 10) to isolate fresh BMMNCs, and the BMMNCs were then cryopreserved for 8 weeks. Both the fresh and the cryopreserved BMMNCs were evaluated for their bioactivity and osteogenic differentiation capacity. In addition, BMMNCs were isolated 2 weeks after SAON induction and subjected to the same evaluations. For in vivo study, cryopreserved BMMNCs were implanted into the bone tunnel during core decompression of the femur (n = 12 rabbits) after the induction of SAON, and tissue regeneration was evaluated by microcomputed tomography and histologic analyses at 12 weeks postoperation. Results. In vitro, there were no significant differences in the bioactivity or ability to undergo osteogenic differentiation between fresh BMMNCs and cryopreserved BMMNCs, but after SAON induction, both features were decreased significantly. In vivo, the bone mineral density, ratio of bone volume to total volume of bone, and volume and diameter of neovascularization within the bone tunnel were significantly higher in the BMMNC-treated group compared to the nontreated control group at 12 weeks postoperation. Conclusion. Cryopreserved BMMNCs maintained their bioactivity and promoted bone regeneration and neovascularization within the bone tunnel after core decompression in this rabbit model of SAON.
UR - https://www.scopus.com/pages/publications/84860442659
UR - https://onlinelibrary.wiley.com/doi/10.1002/art.34525
U2 - 10.1002/art.34525
DO - 10.1002/art.34525
M3 - Journal article
C2 - 22544527
AN - SCOPUS:84860442659
SN - 0004-3591
VL - 64
SP - 1562
EP - 1571
JO - Arthritis and Rheumatism
JF - Arthritis and Rheumatism
IS - 5
ER -
