TY - JOUR
T1 - Profiling of Selected Functional Metabolites in the Central Nervous System of Marine Medaka (Oryzias melastigma) for Environmental Neurotoxicological Assessments
AU - Lei, Elva Ngai Yu
AU - Yau, Man Shan
AU - Yeung, Chi Chung
AU - Murphy, Margaret B.
AU - Wong, Ka Leung
AU - Lam, Michael Hon Wah
N1 - Publisher Copyright:
© 2016, Springer Science+Business Media New York.
PY - 2017/2
Y1 - 2017/2
N2 - The simultaneous profiling of 43 functional metabolites in the brain of the small model vertebrate organism, marine medaka (Oryzais melastigma), has been accomplished via dansyl chloride derivatization and LC–MS/MS quantification. This technique was applied to examine effects of 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47), one of the most abundant polybrominated diphenyl ether flame retardants in the natural environment, on the central nervous system (CNS) of vertebrates. The model teleosts were fed with bioencapsulated Artemia nauplii for up to 21 days. Multivariate statistical analysis has demonstrated that levels of numerous classical neurotransmitters and their metabolites in the CNS of the fish were perturbed even at the early phase of dietary exposure. Subsequent metabolic pathway analysis further implied potential impairment of the arginine and proline metabolism; glycine, serine and threonine metabolism; d-glutamine and d-glutamate metabolism; alanine, aspartate, and glutamate metabolism; valine, leucine, and isoleucine biosynthesis, and the cysteine and methionine metabolism in the brain of the test organism. Our results demonstrate that targeted profiling of functional metabolites in the CNS may shed light on how the various neurological pathways of vertebrates, including humans, are affected by toxicant/stress exposure.
AB - The simultaneous profiling of 43 functional metabolites in the brain of the small model vertebrate organism, marine medaka (Oryzais melastigma), has been accomplished via dansyl chloride derivatization and LC–MS/MS quantification. This technique was applied to examine effects of 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47), one of the most abundant polybrominated diphenyl ether flame retardants in the natural environment, on the central nervous system (CNS) of vertebrates. The model teleosts were fed with bioencapsulated Artemia nauplii for up to 21 days. Multivariate statistical analysis has demonstrated that levels of numerous classical neurotransmitters and their metabolites in the CNS of the fish were perturbed even at the early phase of dietary exposure. Subsequent metabolic pathway analysis further implied potential impairment of the arginine and proline metabolism; glycine, serine and threonine metabolism; d-glutamine and d-glutamate metabolism; alanine, aspartate, and glutamate metabolism; valine, leucine, and isoleucine biosynthesis, and the cysteine and methionine metabolism in the brain of the test organism. Our results demonstrate that targeted profiling of functional metabolites in the CNS may shed light on how the various neurological pathways of vertebrates, including humans, are affected by toxicant/stress exposure.
UR - http://www.scopus.com/inward/record.url?scp=85006368900&partnerID=8YFLogxK
U2 - 10.1007/s00244-016-0342-0
DO - 10.1007/s00244-016-0342-0
M3 - Journal article
C2 - 27990605
AN - SCOPUS:85006368900
SN - 0090-4341
VL - 72
SP - 269
EP - 280
JO - Archives of Environmental Contamination and Toxicology
JF - Archives of Environmental Contamination and Toxicology
IS - 2
ER -