Profiling of Selected Functional Metabolites in the Central Nervous System of Marine Medaka (Oryzias melastigma) for Environmental Neurotoxicological Assessments

Elva Ngai Yu Lei, Man Shan Yau, Chi Chung Yeung, Margaret B. Murphy, Ka-Leung WONG, Michael Hon Wah Lam*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The simultaneous profiling of 43 functional metabolites in the brain of the small model vertebrate organism, marine medaka (Oryzais melastigma), has been accomplished via dansyl chloride derivatization and LC–MS/MS quantification. This technique was applied to examine effects of 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47), one of the most abundant polybrominated diphenyl ether flame retardants in the natural environment, on the central nervous system (CNS) of vertebrates. The model teleosts were fed with bioencapsulated Artemia nauplii for up to 21 days. Multivariate statistical analysis has demonstrated that levels of numerous classical neurotransmitters and their metabolites in the CNS of the fish were perturbed even at the early phase of dietary exposure. Subsequent metabolic pathway analysis further implied potential impairment of the arginine and proline metabolism; glycine, serine and threonine metabolism; d-glutamine and d-glutamate metabolism; alanine, aspartate, and glutamate metabolism; valine, leucine, and isoleucine biosynthesis, and the cysteine and methionine metabolism in the brain of the test organism. Our results demonstrate that targeted profiling of functional metabolites in the CNS may shed light on how the various neurological pathways of vertebrates, including humans, are affected by toxicant/stress exposure.

Original languageEnglish
Pages (from-to)269-280
Number of pages12
JournalArchives of Environmental Contamination and Toxicology
Volume72
Issue number2
DOIs
Publication statusPublished - Feb 2017

Scopus Subject Areas

  • Toxicology
  • Pollution
  • Health, Toxicology and Mutagenesis

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