Production of α-galactosidase by Monascus grown on soybean and sugarcane wastes

Y. L. Wong-Leung, David W F FONG*, W. L. Lam

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Extracts of both sugarcane and soybean wastes supported the growth of Monascus but sugarcane waste was superior for the production of α-galactosidase. An aqueous extract prepared from 5% (w/v) soybean waste and 7% (w/v) sugarcane waste gave the best result and was superior to the standard peptone/glucose/yeast extract medium. Liquid-solid mixtures were slightly less effective. Enzyme production could be enhanced by adding raffinose. Enzymatic hydrolysis of p-nitrophenyl-α-D-galactoside was optimal at pH 4.5. Raffinose and stachyose were hydrolysed to sucrose and galactose.

Original languageEnglish
Pages (from-to)529-533
Number of pages5
JournalWorld Journal of Microbiology and Biotechnology
Volume9
Issue number5
DOIs
Publication statusPublished - Sep 1993

Scopus Subject Areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

User-Defined Keywords

  • α-Galactosidase
  • Monascus
  • soybean waste
  • sugarcane waste

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