Proanthocyanidins Inhibit Seed Germination by Maintaining a High Level of Abscisic Acid in Arabidopsis thaliana

Liguo Jia, Qiuyu Wu, Nenghui Ye, Rui Liu, Lu Shi, Weifeng Xu, Hui Zhi, A. N.M.Rubaiyath Bin Rahman, Yiji XIA, Jianhua ZHANG*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Proanthocyanidins (PAs) are the main products of the flavonoid biosynthetic pathway in seeds, but their biological function during seed germination is still unclear. We observed that seed germination is delayed with the increase of exogenous PA concentration in Arabidopsis. A similar inhibitory effect occurred in peeled Brassica napus seeds, which was observed by measuring radicle elongation. Using abscisic acid (ABA), a biosynthetic and metabolic inhibitor, and gene expression analysis by real-time polymerase chain reaction, we found that the inhibitory effect of PAs on seed germination is due to their promotion of ABA via de novo biogenesis, rather than by any inhibition of its degradation. Consistent with the relationship between PA content and ABA accumulation in seeds, PA-deficient mutants maintain a lower level of ABA compared with wild-types during germination. Our data suggest that PA distribution in the seed coat can act as a doorkeeper to seed germination. PA regulation of seed germination is mediated by the ABA signaling pathway.

Original languageEnglish
Pages (from-to)663-673
Number of pages11
JournalJournal of Integrative Plant Biology
Volume54
Issue number9
DOIs
Publication statusPublished - Sep 2012

Scopus Subject Areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

User-Defined Keywords

  • Abscisic acid
  • Arabidopsis
  • Brassica napus
  • Proanthocyanidins
  • Seed germination

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