Presence of neuroglobin in cultured astrocytes

Qian Chen Xiao; Ye Qin Lu; Gang Zhang Chen; Tao Yang Li; Zhen Gao; Shuang Liu; Lok Ting Lau; Yin Wan Wendy Fung; David A. Greenberg; Albert Cheung Hoi Yu

doi:10.1002/glia.20147

Presence of neuroglobin in cultured astrocytes

Qian Chen Xiao, Ye Qin Lu, Gang Zhang Chen, Tao Yang Li, Zhen Gao, Shuang Liu, Lok Ting Lau, Yin Wan Wendy Fung, David A. Greenberg, Albert Cheung Hoi Yu^*

^*Corresponding author for this work

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Abstract

Neuroglobin (Ngb), a recently discovered intracellular respiratory globin in neurons, may play a crucial role in oxygen homeostasis in the brain. We report preliminary findings indicating the presence of functional neuroglobin in primary cultures of cerebral cortical astrocytes. Reverse transcription real-time polymerase chain reaction (RRT-PCR) and immunostaining confirmed such presence in cultured astrocytes isolated from newborn mouse brain. Ngb antisense treatment increased apoptosis in ischemic astrocytes. The discovery of Ngb in astrocytes may provide some insight into how oxygen homeostasis is regulated in the brain.

Original language	English
Pages (from-to)	182-186
Number of pages	5
Journal	GLIA
Volume	50
Issue number	2
DOIs	https://doi.org/10.1002/glia.20147
Publication status	Published - 15 Apr 2005

Scopus Subject Areas

Neurology
Cellular and Molecular Neuroscience

User-Defined Keywords

Brain
Homeostasis
Neuron
Ngb
Oxygen

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/glia.20147

Cite this

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title = "Presence of neuroglobin in cultured astrocytes",

abstract = "Neuroglobin (Ngb), a recently discovered intracellular respiratory globin in neurons, may play a crucial role in oxygen homeostasis in the brain. We report preliminary findings indicating the presence of functional neuroglobin in primary cultures of cerebral cortical astrocytes. Reverse transcription real-time polymerase chain reaction (RRT-PCR) and immunostaining confirmed such presence in cultured astrocytes isolated from newborn mouse brain. Ngb antisense treatment increased apoptosis in ischemic astrocytes. The discovery of Ngb in astrocytes may provide some insight into how oxygen homeostasis is regulated in the brain.",

keywords = "Brain, Homeostasis, Neuron, Ngb, Oxygen",

author = "Xiao, {Qian Chen} and Lu, {Ye Qin} and Chen, {Gang Zhang} and Li, {Tao Yang} and Zhen Gao and Shuang Liu and Lau, {Lok Ting} and Fung, {Yin Wan Wendy} and Greenberg, {David A.} and Yu, {Albert Cheung Hoi}",

note = "Natural Science Foundation of China. Grant Numbers: 30270426, 30470543 Beijing National Science Foundation. Grant Numbers: 703026, 7051004 Publisher Copyright: {\textcopyright} 2005 Wiley-Liss, Inc.",

year = "2005",

month = apr,

day = "15",

doi = "10.1002/glia.20147",

language = "English",

volume = "50",

pages = "182--186",

journal = "GLIA",

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T1 - Presence of neuroglobin in cultured astrocytes

AU - Xiao, Qian Chen

AU - Lu, Ye Qin

AU - Chen, Gang Zhang

AU - Li, Tao Yang

AU - Gao, Zhen

AU - Liu, Shuang

AU - Lau, Lok Ting

AU - Fung, Yin Wan Wendy

AU - Greenberg, David A.

AU - Yu, Albert Cheung Hoi

PY - 2005/4/15

Y1 - 2005/4/15

N2 - Neuroglobin (Ngb), a recently discovered intracellular respiratory globin in neurons, may play a crucial role in oxygen homeostasis in the brain. We report preliminary findings indicating the presence of functional neuroglobin in primary cultures of cerebral cortical astrocytes. Reverse transcription real-time polymerase chain reaction (RRT-PCR) and immunostaining confirmed such presence in cultured astrocytes isolated from newborn mouse brain. Ngb antisense treatment increased apoptosis in ischemic astrocytes. The discovery of Ngb in astrocytes may provide some insight into how oxygen homeostasis is regulated in the brain.

AB - Neuroglobin (Ngb), a recently discovered intracellular respiratory globin in neurons, may play a crucial role in oxygen homeostasis in the brain. We report preliminary findings indicating the presence of functional neuroglobin in primary cultures of cerebral cortical astrocytes. Reverse transcription real-time polymerase chain reaction (RRT-PCR) and immunostaining confirmed such presence in cultured astrocytes isolated from newborn mouse brain. Ngb antisense treatment increased apoptosis in ischemic astrocytes. The discovery of Ngb in astrocytes may provide some insight into how oxygen homeostasis is regulated in the brain.

KW - Brain

KW - Homeostasis

KW - Neuron

KW - Ngb

KW - Oxygen

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UR - https://onlinelibrary.wiley.com/doi/10.1002/glia.20147

U2 - 10.1002/glia.20147

DO - 10.1002/glia.20147

M3 - Journal article

C2 - 15657899

AN - SCOPUS:18244386984

SN - 0894-1491

VL - 50

SP - 182

EP - 186

JO - GLIA

JF - GLIA

IS - 2

ER -

Presence of neuroglobin in cultured astrocytes

Abstract

Scopus Subject Areas

User-Defined Keywords

UN SDGs

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