Preparative separation of gambogic acid and its C-2 epimer using recycling high-speed counter-current chromatography

Quan Bin Han; Jing Zheng Song; Chun Feng Qiao; Lina Wong; Hong Xi Xu

doi:10.1016/j.chroma.2006.07.044

Preparative separation of gambogic acid and its C-2 epimer using recycling high-speed counter-current chromatography

Quan Bin Han, Jing Zheng Song, Chun Feng Qiao, Lina Wong, Hong Xi Xu^*

^*Corresponding author for this work

Chinese Medicine - Teaching and Research Division

Research output: Contribution to journal › Journal article › peer-review

82 Citations (Scopus)

Abstract

A recycling counter-current chromatographic system was first set up with a high-speed counter-current chromatography instrument coupled with a column switching valve. This system was first successfully applied to the preparative separation of epimers, gambogic acid and epigambogic acid from Garcinia hanburyi using n-hexane-methanol-water (5:4:1, v/v/v) as the two-phase solvent system. As a result, 28.2 mg gambogic acid and 18.4 mg epigambogic acid were separated from 50 mg of mixture. Their purities were both above 97% as determined by HPLC. The chemical structures were then identified by their ¹H NMR and ¹³C NMR spectra.

Original language	English
Pages (from-to)	298-301
Number of pages	4
Journal	Journal of Chromatography A
Volume	1127
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chroma.2006.07.044
Publication status	Published - 15 Sept 2006

Scopus Subject Areas

Analytical Chemistry
Biochemistry
Organic Chemistry

User-Defined Keywords

Epigambogic acid
Epimer
Gambogic acid
Garcinia hanburyi
Preparative separation
Recycling counter-current chromatography

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10.1016/j.chroma.2006.07.044

Cite this

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title = "Preparative separation of gambogic acid and its C-2 epimer using recycling high-speed counter-current chromatography",

abstract = "A recycling counter-current chromatographic system was first set up with a high-speed counter-current chromatography instrument coupled with a column switching valve. This system was first successfully applied to the preparative separation of epimers, gambogic acid and epigambogic acid from Garcinia hanburyi using n-hexane-methanol-water (5:4:1, v/v/v) as the two-phase solvent system. As a result, 28.2 mg gambogic acid and 18.4 mg epigambogic acid were separated from 50 mg of mixture. Their purities were both above 97% as determined by HPLC. The chemical structures were then identified by their 1H NMR and 13C NMR spectra.",

keywords = "Epigambogic acid, Epimer, Gambogic acid, Garcinia hanburyi, Preparative separation, Recycling counter-current chromatography",

author = "Han, {Quan Bin} and Song, {Jing Zheng} and Qiao, {Chun Feng} and Lina Wong and Xu, {Hong Xi}",

note = "Funding Information: This research is funded by the Hong Kong Jockey Club Charities Trust.",

year = "2006",

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doi = "10.1016/j.chroma.2006.07.044",

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T1 - Preparative separation of gambogic acid and its C-2 epimer using recycling high-speed counter-current chromatography

AU - Han, Quan Bin

AU - Song, Jing Zheng

AU - Qiao, Chun Feng

AU - Wong, Lina

AU - Xu, Hong Xi

N1 - Funding Information: This research is funded by the Hong Kong Jockey Club Charities Trust.

PY - 2006/9/15

Y1 - 2006/9/15

N2 - A recycling counter-current chromatographic system was first set up with a high-speed counter-current chromatography instrument coupled with a column switching valve. This system was first successfully applied to the preparative separation of epimers, gambogic acid and epigambogic acid from Garcinia hanburyi using n-hexane-methanol-water (5:4:1, v/v/v) as the two-phase solvent system. As a result, 28.2 mg gambogic acid and 18.4 mg epigambogic acid were separated from 50 mg of mixture. Their purities were both above 97% as determined by HPLC. The chemical structures were then identified by their 1H NMR and 13C NMR spectra.

AB - A recycling counter-current chromatographic system was first set up with a high-speed counter-current chromatography instrument coupled with a column switching valve. This system was first successfully applied to the preparative separation of epimers, gambogic acid and epigambogic acid from Garcinia hanburyi using n-hexane-methanol-water (5:4:1, v/v/v) as the two-phase solvent system. As a result, 28.2 mg gambogic acid and 18.4 mg epigambogic acid were separated from 50 mg of mixture. Their purities were both above 97% as determined by HPLC. The chemical structures were then identified by their 1H NMR and 13C NMR spectra.

KW - Epigambogic acid

KW - Epimer

KW - Gambogic acid

KW - Garcinia hanburyi

KW - Preparative separation

KW - Recycling counter-current chromatography

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U2 - 10.1016/j.chroma.2006.07.044

DO - 10.1016/j.chroma.2006.07.044

M3 - Journal article

SN - 0021-9673

VL - 1127

SP - 298

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JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 1-2

ER -

Preparative separation of gambogic acid and its C-2 epimer using recycling high-speed counter-current chromatography

Abstract

Scopus Subject Areas

User-Defined Keywords

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