Preparative separation and purification of squalene from the microalga Thraustochytrium ATCC 26185 by high-speed counter-current chromatography

Hai Tao Lu; Yue Jiang; Feng Chen

doi:10.1016/S0021-9673(03)00454-0

Preparative separation and purification of squalene from the microalga Thraustochytrium ATCC 26185 by high-speed counter-current chromatography

Hai Tao Lu, Yue Jiang, Feng Chen^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

48 Citations (Scopus)

Abstract

High-speed counter-current chromatography (HSCCC) was successfully applied to the preparative separation and purification of squalene from microalgae. Crude squalene was obtained from the microalga Thraustochytrium ATCC 26185 by extraction with organic solvents. The crude squalene was further separated using a waterless two-phase solvent system composed of n-hexane-methanol (2:1, v/v). The upper phase as the mobile phase was pumped into the column at a flow-rate of 2.0 ml min^-1 in the tail-to-head elution mode. The fractions purified and collected were analyzed by high-performance liquid chromatography. The method yielded 0.2 mg squalene at 96% purity from 150 mg of the crude squalene (0.14% squalene) with 95% recovery. The separation of squalene by HSCCC was completed in 90 min.

Original language	English
Pages (from-to)	37-43
Number of pages	7
Journal	Journal of Chromatography A
Volume	994
Issue number	1-2
DOIs	https://doi.org/10.1016/S0021-9673(03)00454-0
Publication status	Published - 25 Apr 2003
Externally published	Yes

Scopus Subject Areas

Analytical Chemistry
Biochemistry
Organic Chemistry

User-Defined Keywords

Counter-current chromatography
Squalene
Terpenes
Thraustochytrium ATCC 26185

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10.1016/S0021-9673(03)00454-0

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title = "Preparative separation and purification of squalene from the microalga Thraustochytrium ATCC 26185 by high-speed counter-current chromatography",

abstract = "High-speed counter-current chromatography (HSCCC) was successfully applied to the preparative separation and purification of squalene from microalgae. Crude squalene was obtained from the microalga Thraustochytrium ATCC 26185 by extraction with organic solvents. The crude squalene was further separated using a waterless two-phase solvent system composed of n-hexane-methanol (2:1, v/v). The upper phase as the mobile phase was pumped into the column at a flow-rate of 2.0 ml min-1 in the tail-to-head elution mode. The fractions purified and collected were analyzed by high-performance liquid chromatography. The method yielded 0.2 mg squalene at 96% purity from 150 mg of the crude squalene (0.14% squalene) with 95% recovery. The separation of squalene by HSCCC was completed in 90 min.",

keywords = "Counter-current chromatography, Squalene, Terpenes, Thraustochytrium ATCC 26185",

author = "Lu, {Hai Tao} and Yue Jiang and Feng Chen",

note = "Funding Information: This research was supported by the FRG (Faculty Research Grant) of Hong Kong Baptist University and the RGC (the Research Grants Council of Hong Kong).",

year = "2003",

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doi = "10.1016/S0021-9673(03)00454-0",

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T1 - Preparative separation and purification of squalene from the microalga Thraustochytrium ATCC 26185 by high-speed counter-current chromatography

AU - Lu, Hai Tao

AU - Jiang, Yue

AU - Chen, Feng

N1 - Funding Information: This research was supported by the FRG (Faculty Research Grant) of Hong Kong Baptist University and the RGC (the Research Grants Council of Hong Kong).

PY - 2003/4/25

Y1 - 2003/4/25

N2 - High-speed counter-current chromatography (HSCCC) was successfully applied to the preparative separation and purification of squalene from microalgae. Crude squalene was obtained from the microalga Thraustochytrium ATCC 26185 by extraction with organic solvents. The crude squalene was further separated using a waterless two-phase solvent system composed of n-hexane-methanol (2:1, v/v). The upper phase as the mobile phase was pumped into the column at a flow-rate of 2.0 ml min-1 in the tail-to-head elution mode. The fractions purified and collected were analyzed by high-performance liquid chromatography. The method yielded 0.2 mg squalene at 96% purity from 150 mg of the crude squalene (0.14% squalene) with 95% recovery. The separation of squalene by HSCCC was completed in 90 min.

AB - High-speed counter-current chromatography (HSCCC) was successfully applied to the preparative separation and purification of squalene from microalgae. Crude squalene was obtained from the microalga Thraustochytrium ATCC 26185 by extraction with organic solvents. The crude squalene was further separated using a waterless two-phase solvent system composed of n-hexane-methanol (2:1, v/v). The upper phase as the mobile phase was pumped into the column at a flow-rate of 2.0 ml min-1 in the tail-to-head elution mode. The fractions purified and collected were analyzed by high-performance liquid chromatography. The method yielded 0.2 mg squalene at 96% purity from 150 mg of the crude squalene (0.14% squalene) with 95% recovery. The separation of squalene by HSCCC was completed in 90 min.

KW - Counter-current chromatography

KW - Squalene

KW - Terpenes

KW - Thraustochytrium ATCC 26185

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DO - 10.1016/S0021-9673(03)00454-0

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AN - SCOPUS:0038667517

SN - 0021-9673

VL - 994

SP - 37

EP - 43

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 1-2

ER -

Preparative separation and purification of squalene from the microalga Thraustochytrium ATCC 26185 by high-speed counter-current chromatography

Abstract

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