Preparative separation and purification of squalene from the microalga Thraustochytrium ATCC 26185 by high-speed counter-current chromatography

Hai Tao Lu, Yue Jiang, Feng Chen*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

47 Citations (Scopus)

Abstract

High-speed counter-current chromatography (HSCCC) was successfully applied to the preparative separation and purification of squalene from microalgae. Crude squalene was obtained from the microalga Thraustochytrium ATCC 26185 by extraction with organic solvents. The crude squalene was further separated using a waterless two-phase solvent system composed of n-hexane-methanol (2:1, v/v). The upper phase as the mobile phase was pumped into the column at a flow-rate of 2.0 ml min-1 in the tail-to-head elution mode. The fractions purified and collected were analyzed by high-performance liquid chromatography. The method yielded 0.2 mg squalene at 96% purity from 150 mg of the crude squalene (0.14% squalene) with 95% recovery. The separation of squalene by HSCCC was completed in 90 min.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalJournal of Chromatography A
Volume994
Issue number1-2
DOIs
Publication statusPublished - 25 Apr 2003
Externally publishedYes

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

User-Defined Keywords

  • Counter-current chromatography
  • Squalene
  • Terpenes
  • Thraustochytrium ATCC 26185

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