Preparative isolation of Pseudolaric acids A and B, and their glucosides from the root bark of Pseudolarix kaempferi using high-speed counter-current chromatography

Quan Bin Han, Lina Wong, Fanny Lai, Nian Yun Yang, Jing Zheng Song, Chun Feng Qiao, Hong Xi Xu*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

8 Citations (Scopus)

Abstract

In order to provide the chemical markers for the quality control of herbal medicines, four diterpenoids, pseudolaric acids A and B (PAA and PAB), and their glucosides were isolated from the methanol extract of the Chinese herb Pseudolarix kaempferi using high-speed counter-current chromatography (HSCCC). The diphase solvent system was n-hexane/EtOAc/ MeOH/H2O which was used at two ratios (5:5:5:5 and 1:9:4:6 by volume) in the separation of pseudolaric acids and their glycosides, respectively. As a result, PAA (14 mg), PAB (129 mg), PAA-O-β-D-glucopyranoside (8 mg, PAAG), and PAB-O-β-D-glucopyranoside (42 mg, PABG) were obtained from 0.5 g of the crude extract. Their purities were determined to be above 97% by HPLC analysis. Their chemical structures were confirmed by 1H and 13C NMR analysis or HPLC comparison with the reference compounds.

Original languageEnglish
Pages (from-to)309-313
Number of pages5
JournalJournal of Separation Science
Volume32
Issue number2
DOIs
Publication statusPublished - Jan 2009

Scopus Subject Areas

  • Analytical Chemistry
  • Filtration and Separation

User-Defined Keywords

  • High-speed counter-current chromatography
  • Preparative isolation
  • Pseudolaric acid A
  • Pseudolaric acid B
  • Pseudolarix kaempferi

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