Abstract
As a chromatographic column, the high-speed counter-current chromatography system was equipped with a preparative HPLC series, enabling the successful isolation of hydrolysable tannins from the fruits of Terminalia chebula, a traditional Chinese medicine. The two-phase solvent system was composed of n-hexane-ethyl acetate-methanol-water (1:20:1:20 v/v). As a result, 33.2 mg chebulagic and 15.8 mg chebulinic acids were obtained in one step from 300 mg of crude extract. Their purities were determined by HPLC to be 95.3 and 96.1%, respectively. The chemical structures were identified by their MS and 1H NMR spectra.
Original language | English |
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Pages (from-to) | 1653-1657 |
Number of pages | 5 |
Journal | Journal of Separation Science |
Volume | 29 |
Issue number | 11 |
DOIs | |
Publication status | Published - 17 Jul 2006 |
Scopus Subject Areas
- Analytical Chemistry
- Filtration and Separation
User-Defined Keywords
- Chebulagic acid
- Chebulinic acid
- Counter-current chromatography
- Hydrolyzable tannin
- Terminalia chebula