Preparative isolation of hydrolysable tannins chebulagic acid and chebulinic acid from Terminalia chebula by high-speed counter-current chromatography

Quanbin Han, Jingzheng Song, Chunfeng Qiao, Lina Wong, Hongxi Xu

Research output: Contribution to journalJournal articlepeer-review

68 Citations (Scopus)

Abstract

As a chromatographic column, the high-speed counter-current chromatography system was equipped with a preparative HPLC series, enabling the successful isolation of hydrolysable tannins from the fruits of Terminalia chebula, a traditional Chinese medicine. The two-phase solvent system was composed of n-hexane-ethyl acetate-methanol-water (1:20:1:20 v/v). As a result, 33.2 mg chebulagic and 15.8 mg chebulinic acids were obtained in one step from 300 mg of crude extract. Their purities were determined by HPLC to be 95.3 and 96.1%, respectively. The chemical structures were identified by their MS and 1H NMR spectra.

Original languageEnglish
Pages (from-to)1653-1657
Number of pages5
JournalJournal of Separation Science
Volume29
Issue number11
DOIs
Publication statusPublished - 17 Jul 2006

Scopus Subject Areas

  • Analytical Chemistry
  • Filtration and Separation

User-Defined Keywords

  • Chebulagic acid
  • Chebulinic acid
  • Counter-current chromatography
  • Hydrolyzable tannin
  • Terminalia chebula

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