TY - JOUR
T1 - Preparative isolation of cyclolanostane-type saponins from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by TLC-MS/MS guided high-speed counter-current chromatography
AU - HAN, Simon Q B
AU - Song, Jingzheng
AU - Qiao, Chunfeng
AU - Wong, Lina
AU - Xu, Hongxi
PY - 2007/1
Y1 - 2007/1
N2 - Two cyclolanostane-type saponins, astragalosides I and II, were first identified by TLC-MS/MS in the ethyl acetate extract of the roots of Astragalus membranaceus Bge var. mongholicus (Bge.) Hsiao without chemical reference substances. They were then isolated by high-speed counter-current chromatography with a two-step two-phase solvent system of ethyl acetate-2-propanol–water (5:1:5, 50:1:50, v/v/v). The quantities of astragalosides I and II isolated from 1 g of the crude extract were 30.2 mg and 16.5 mg, respectively. Their purities were found to be over 95% by HPLC–ELSD analysis. Their chemical structures were confirmed by 1H and 13C nuclear magnetic resonance analysis.
AB - Two cyclolanostane-type saponins, astragalosides I and II, were first identified by TLC-MS/MS in the ethyl acetate extract of the roots of Astragalus membranaceus Bge var. mongholicus (Bge.) Hsiao without chemical reference substances. They were then isolated by high-speed counter-current chromatography with a two-step two-phase solvent system of ethyl acetate-2-propanol–water (5:1:5, 50:1:50, v/v/v). The quantities of astragalosides I and II isolated from 1 g of the crude extract were 30.2 mg and 16.5 mg, respectively. Their purities were found to be over 95% by HPLC–ELSD analysis. Their chemical structures were confirmed by 1H and 13C nuclear magnetic resonance analysis.
UR - https://pubmed.ncbi.nlm.nih.gov/17313153/#affiliation-1
U2 - 10.1002/jssc.200600264
DO - 10.1002/jssc.200600264
M3 - Journal article
SN - 1615-9306
VL - 30
SP - 135
EP - 140
JO - Journal of Separation Science
JF - Journal of Separation Science
IS - 1
ER -