Preparation of gold nanoparticles on eggshell membrane and their biosensing application

Baozhan Zheng, Lei Qian, Hongyan Yuan, Dan Xiao*, Xiupei Yang, Man Chin Paau, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

101 Citations (Scopus)


A facile green biosynthesis method has been successfully developed to prepare gold nanoparticles (AuNPs) of various core sizes (25 ± 7 nm) using a natural biomaterial, eggshell membrane (ESM) at ambient conditions. In situ synthesis of AuNPs-immobilized ESM is conducted in a simple manner by immersing ESM in a pH 6.0 aqueous solution of HAuCl4 without adding any reductant. The formation of AuNPs on ESM protein fibers is attributed to the reduction of Au(III) ions to Au(0) by the aldehyde moieties of the natural ESM fibers. Energy dispersive X-ray spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray powder diffraction unambiguously identify the presence of AuNPs on ESM. The effect of pH on the in situ synthesis of AuNPs on ESM has been investigated in detail. The pH of the gold precursor (HAuCl4) solution can influence the formation rate, dispersion and size of AuNPs on ESM. At pH ≤3.0 and ≥7.0, no AuNPs are observed on ESM while small AuNPs are homogeneously dispersed on ESM at pH 4.0-6.0. The optimal pH for AuNPs formation on ESM is 6.0. AuNPs/ESMs are used to immobilize glucose oxidase (GOx) for glucose biosensing. AuNPs on ESM can increase the enzyme activity of GOx. The linear response range of the glucose biosensor is 20 μM to 0.80 mM glucose with a detection limit of 17 μM (S/N = 3). The biosensor has been successfully applied to determine the glucose content in commercial glucose injections. Our work provides a very simple, non-toxic, convenient, and green route to synthesize AuNPs on ESM which is potentially useful in the biosensing field.

Original languageEnglish
Pages (from-to)177-183
Number of pages7
Issue number1
Publication statusPublished - 30 Jun 2010

Scopus Subject Areas

  • Analytical Chemistry

User-Defined Keywords

  • Biocatalysis
  • Eggshell membrane
  • Glucose biosensors
  • Gold nanoparticles


Dive into the research topics of 'Preparation of gold nanoparticles on eggshell membrane and their biosensing application'. Together they form a unique fingerprint.

Cite this