Preparation of gold nanoparticles on eggshell membrane and their biosensing application

Baozhan Zheng; Lei Qian; Hongyan Yuan; Dan Xiao; Xiupei Yang; Man Chin Paau; Martin M.F. Choi

doi:10.1016/j.talanta.2010.04.014

Preparation of gold nanoparticles on eggshell membrane and their biosensing application

Baozhan Zheng, Lei Qian, Hongyan Yuan, Dan Xiao^*, Xiupei Yang, Man Chin Paau, Martin M.F. Choi

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

105 Citations (Scopus)

Abstract

A facile green biosynthesis method has been successfully developed to prepare gold nanoparticles (AuNPs) of various core sizes (25 ± 7 nm) using a natural biomaterial, eggshell membrane (ESM) at ambient conditions. In situ synthesis of AuNPs-immobilized ESM is conducted in a simple manner by immersing ESM in a pH 6.0 aqueous solution of HAuCl₄ without adding any reductant. The formation of AuNPs on ESM protein fibers is attributed to the reduction of Au(III) ions to Au(0) by the aldehyde moieties of the natural ESM fibers. Energy dispersive X-ray spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray powder diffraction unambiguously identify the presence of AuNPs on ESM. The effect of pH on the in situ synthesis of AuNPs on ESM has been investigated in detail. The pH of the gold precursor (HAuCl₄) solution can influence the formation rate, dispersion and size of AuNPs on ESM. At pH ≤3.0 and ≥7.0, no AuNPs are observed on ESM while small AuNPs are homogeneously dispersed on ESM at pH 4.0-6.0. The optimal pH for AuNPs formation on ESM is 6.0. AuNPs/ESMs are used to immobilize glucose oxidase (GO_x) for glucose biosensing. AuNPs on ESM can increase the enzyme activity of GO_x. The linear response range of the glucose biosensor is 20 μM to 0.80 mM glucose with a detection limit of 17 μM (S/N = 3). The biosensor has been successfully applied to determine the glucose content in commercial glucose injections. Our work provides a very simple, non-toxic, convenient, and green route to synthesize AuNPs on ESM which is potentially useful in the biosensing field.

Original language	English
Pages (from-to)	177-183
Number of pages	7
Journal	Talanta
Volume	82
Issue number	1
DOIs	https://doi.org/10.1016/j.talanta.2010.04.014
Publication status	Published - 30 Jun 2010

Scopus Subject Areas

Analytical Chemistry

User-Defined Keywords

Biocatalysis
Eggshell membrane
Glucose biosensors
Gold nanoparticles

Access to Document

10.1016/j.talanta.2010.04.014

Cite this

@article{6ede6b562cb241d7805b6fb4256903ce,

title = "Preparation of gold nanoparticles on eggshell membrane and their biosensing application",

abstract = "A facile green biosynthesis method has been successfully developed to prepare gold nanoparticles (AuNPs) of various core sizes (25 ± 7 nm) using a natural biomaterial, eggshell membrane (ESM) at ambient conditions. In situ synthesis of AuNPs-immobilized ESM is conducted in a simple manner by immersing ESM in a pH 6.0 aqueous solution of HAuCl4 without adding any reductant. The formation of AuNPs on ESM protein fibers is attributed to the reduction of Au(III) ions to Au(0) by the aldehyde moieties of the natural ESM fibers. Energy dispersive X-ray spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray powder diffraction unambiguously identify the presence of AuNPs on ESM. The effect of pH on the in situ synthesis of AuNPs on ESM has been investigated in detail. The pH of the gold precursor (HAuCl4) solution can influence the formation rate, dispersion and size of AuNPs on ESM. At pH ≤3.0 and ≥7.0, no AuNPs are observed on ESM while small AuNPs are homogeneously dispersed on ESM at pH 4.0-6.0. The optimal pH for AuNPs formation on ESM is 6.0. AuNPs/ESMs are used to immobilize glucose oxidase (GOx) for glucose biosensing. AuNPs on ESM can increase the enzyme activity of GOx. The linear response range of the glucose biosensor is 20 μM to 0.80 mM glucose with a detection limit of 17 μM (S/N = 3). The biosensor has been successfully applied to determine the glucose content in commercial glucose injections. Our work provides a very simple, non-toxic, convenient, and green route to synthesize AuNPs on ESM which is potentially useful in the biosensing field.",

keywords = "Biocatalysis, Eggshell membrane, Glucose biosensors, Gold nanoparticles",

author = "Baozhan Zheng and Lei Qian and Hongyan Yuan and Dan Xiao and Xiupei Yang and Paau, {Man Chin} and Choi, {Martin M.F.}",

note = "Funding Information: We would express our sincere thanks to Mr. Tommy W.H. Poon of the Department of Chemistry, Hong Kong Baptist University for acquiring the SEM images and EDS, and Ms. Y.K. Wu of the Centre for Surface Analysis and Research, Hong Kong Baptist University for the XPS and XRD measurements. Financial support from the Research Grants Council of the Hong Kong Special Administrative Region, China (project no. HKBU 200907) is gratefully acknowledged. Appendix A ",

year = "2010",

month = jun,

day = "30",

doi = "10.1016/j.talanta.2010.04.014",

language = "English",

volume = "82",

pages = "177--183",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Preparation of gold nanoparticles on eggshell membrane and their biosensing application

AU - Zheng, Baozhan

AU - Qian, Lei

AU - Yuan, Hongyan

AU - Xiao, Dan

AU - Yang, Xiupei

AU - Paau, Man Chin

AU - Choi, Martin M.F.

N1 - Funding Information: We would express our sincere thanks to Mr. Tommy W.H. Poon of the Department of Chemistry, Hong Kong Baptist University for acquiring the SEM images and EDS, and Ms. Y.K. Wu of the Centre for Surface Analysis and Research, Hong Kong Baptist University for the XPS and XRD measurements. Financial support from the Research Grants Council of the Hong Kong Special Administrative Region, China (project no. HKBU 200907) is gratefully acknowledged. Appendix A

PY - 2010/6/30

Y1 - 2010/6/30

N2 - A facile green biosynthesis method has been successfully developed to prepare gold nanoparticles (AuNPs) of various core sizes (25 ± 7 nm) using a natural biomaterial, eggshell membrane (ESM) at ambient conditions. In situ synthesis of AuNPs-immobilized ESM is conducted in a simple manner by immersing ESM in a pH 6.0 aqueous solution of HAuCl4 without adding any reductant. The formation of AuNPs on ESM protein fibers is attributed to the reduction of Au(III) ions to Au(0) by the aldehyde moieties of the natural ESM fibers. Energy dispersive X-ray spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray powder diffraction unambiguously identify the presence of AuNPs on ESM. The effect of pH on the in situ synthesis of AuNPs on ESM has been investigated in detail. The pH of the gold precursor (HAuCl4) solution can influence the formation rate, dispersion and size of AuNPs on ESM. At pH ≤3.0 and ≥7.0, no AuNPs are observed on ESM while small AuNPs are homogeneously dispersed on ESM at pH 4.0-6.0. The optimal pH for AuNPs formation on ESM is 6.0. AuNPs/ESMs are used to immobilize glucose oxidase (GOx) for glucose biosensing. AuNPs on ESM can increase the enzyme activity of GOx. The linear response range of the glucose biosensor is 20 μM to 0.80 mM glucose with a detection limit of 17 μM (S/N = 3). The biosensor has been successfully applied to determine the glucose content in commercial glucose injections. Our work provides a very simple, non-toxic, convenient, and green route to synthesize AuNPs on ESM which is potentially useful in the biosensing field.

AB - A facile green biosynthesis method has been successfully developed to prepare gold nanoparticles (AuNPs) of various core sizes (25 ± 7 nm) using a natural biomaterial, eggshell membrane (ESM) at ambient conditions. In situ synthesis of AuNPs-immobilized ESM is conducted in a simple manner by immersing ESM in a pH 6.0 aqueous solution of HAuCl4 without adding any reductant. The formation of AuNPs on ESM protein fibers is attributed to the reduction of Au(III) ions to Au(0) by the aldehyde moieties of the natural ESM fibers. Energy dispersive X-ray spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray powder diffraction unambiguously identify the presence of AuNPs on ESM. The effect of pH on the in situ synthesis of AuNPs on ESM has been investigated in detail. The pH of the gold precursor (HAuCl4) solution can influence the formation rate, dispersion and size of AuNPs on ESM. At pH ≤3.0 and ≥7.0, no AuNPs are observed on ESM while small AuNPs are homogeneously dispersed on ESM at pH 4.0-6.0. The optimal pH for AuNPs formation on ESM is 6.0. AuNPs/ESMs are used to immobilize glucose oxidase (GOx) for glucose biosensing. AuNPs on ESM can increase the enzyme activity of GOx. The linear response range of the glucose biosensor is 20 μM to 0.80 mM glucose with a detection limit of 17 μM (S/N = 3). The biosensor has been successfully applied to determine the glucose content in commercial glucose injections. Our work provides a very simple, non-toxic, convenient, and green route to synthesize AuNPs on ESM which is potentially useful in the biosensing field.

KW - Biocatalysis

KW - Eggshell membrane

KW - Glucose biosensors

KW - Gold nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=77955427569&partnerID=8YFLogxK

U2 - 10.1016/j.talanta.2010.04.014

DO - 10.1016/j.talanta.2010.04.014

M3 - Journal article

AN - SCOPUS:77955427569

SN - 0039-9140

VL - 82

SP - 177

EP - 183

JO - Talanta

JF - Talanta

IS - 1

ER -

Preparation of gold nanoparticles on eggshell membrane and their biosensing application

Abstract

Scopus Subject Areas

User-Defined Keywords

Access to Document

Other files and links

Fingerprint

Cite this