Preparation of DNA from Arabidopsis

Jianming Li*, Joanne Chory

*Corresponding author for this work

Research output: Chapter in book/report/conference proceedingChapterpeer-review

31 Citations (Scopus)

Abstract

Successful extraction of DNA is an essential and time consuming step in many plant molecular biology procedures. The classical approaches of plant DNA isolation are often designed to produce large amounts of DNA of high molecular weight with sufficient purity. Since the polymerase chain reaction (PCR) 1s the method of choice in modern plant genetic analyses such as map-based breeding and positional gene clonmg, many recently developed protocols are aimed at rapid extraction of a minute quantity of plant DNA that is suitable for PCR. This allows large numbers of samples to be analyzed m a short period of time.
Original languageEnglish
Title of host publicationArabidopsis Protocols
EditorsJosé M. Martinez-Zapater, Julio Salinas
PublisherHumana Press
Pages55-60
Number of pages6
Edition1st
ISBN (Electronic)9781592592685
DOIs
Publication statusPublished - Jan 1998

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
Volume82
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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