Preparation and quantification of the total phenolic products in Citrus fruit using solid-phase extraction coupled with high-performance liquid chromatography with diode array and UV detection

Honglian Zeng, Zhenli Liu, Siyu Zhao, Yisong Shu, Zhiqian Song, Chun Wang, Yunzhuo Dong, Zhangchi Ning, Dan He, Menglei Wang, Cheng Lu, Yuanyan Liu*, Aiping LYU

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Citrus fruit is an important health-promoting food that is rich in dietary phenolic metabolites. Traditional Chinese medicines, such as Zhishi and Zhiqiao, come from young and immature fruits of Citrus cultivars. The preparation of diversified bioactive phenolic products and establishment of the corresponding quality control methodology are challenging and necessary. In the current study, four types of solid-phase extraction sorbents for the enrichment and clean-up of the phenolic matrix were evaluated. A solid-phase extraction column coated with Strata-X was finally used in the procedure. Twenty phenolic compounds were selected to evaluate the extraction performances of the sorbents using high-performance liquid chromatography analysis. Under the optimized conditions, good linearities were obtained with R2 more than 0.9996 for all analytes with LODs of 0.04–1.012 μg/g. Intra- and interday relative standard deviation values were less than 3%, and the recovery was equal to or higher than 90.02%. Compared to non-solid-phase extraction process, the content of total phenolic products was elevated 35.55–68.48% with solid-phase extraction. Finally, the developed and validated method was successfully applied to the discrimination of Zhishi samples from different species as well as Zhishi and Zhiqiao samples in different development stages.

Original languageEnglish
Pages (from-to)3806-3817
Number of pages12
JournalJournal of Separation Science
Volume39
Issue number19
DOIs
Publication statusPublished - 1 Oct 2016

Scopus Subject Areas

  • Analytical Chemistry
  • Filtration and Separation

User-Defined Keywords

  • Quality control
  • Solid-phase extraction
  • Total phenolic products
  • Zhiqiao
  • Zhishi

Fingerprint

Dive into the research topics of 'Preparation and quantification of the total phenolic products in Citrus fruit using solid-phase extraction coupled with high-performance liquid chromatography with diode array and UV detection'. Together they form a unique fingerprint.

Cite this