Potential cytotoxicity of water-soluble fraction of dust and particulate matters and relation to metal(loid)s based on three human cell lines

Minjuan Huang*, Yuan Kang, Wei Wang, Chuen Yu Chan, Xuemei Wang, Ming Hung Wong

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

31 Citations (Scopus)

Abstract

Hepatocellular liver carcinoma (HepG2), human skin derived keratinocyte (KERTr,) and lung epithelial carcinoma (A549) were employed in MTT assay to evaluate the cytotoxicity of water-soluble fraction of road dust, air-conditioning (AC) filter dust and PM2.5 via ingestion, dermal contact and inhalation. Their effects on cell growth were dependent on exposure time and concentration. The LC20s of PM2.5 for A549 cell were approximately one order of magnitude lower than those of road dust and AC filter dust for KERTr cell and HepG2 cell. The LC20s of aqueous extracts were negatively correlated to the water-soluble metal(loid)s contained in dust coarse particles (KERTr: p=0.004; HepG2: p<0.001). However, no significant correlation between soluble metal(loid)s and LC20s of PM2.5 was observed for A549 cell (p>0.05). Other water-soluble components in dust and PM might cause the cell hazards synergistically or additively with metal(loid)s.

Original languageEnglish
Pages (from-to)61-66
Number of pages6
JournalChemosphere
Volume135
DOIs
Publication statusPublished - 1 Sept 2015

Scopus Subject Areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

User-Defined Keywords

  • A549
  • Cytotoxicity
  • Dust and PM
  • HepG2
  • KERTr
  • Water-soluble metal(loid)s

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