Polysaccharides of Dendrobium officinale inhibit TNF-α-induced apoptosis in A-253 cell line

L. Xiang, C. W. Stephen Sze, T. B. Ng, Y. Tong, P. C. Shaw, C. W. Sydney Tang, Y. B. Kalin Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Objective: Our previous study demonstrated that polysaccharides of Dendrobium officinale Kimura et Migo (DP) were capable of enhancing immunomodulation in an experimental model of Sjögren's syndrome, a chronic autoimmune disease mainly affecting the salivary glands. In the present study, we further investigated the protective effect of DP on a human salivary gland cell line A-253 against tumor necrosis factor (TNF)-α-induced apoptosis. 

Materials: TNF-α (100 U/ml) was used as the stimulus for treating the A-253 cells to induce cellular apoptosis. Nuclear factor-kappa B (NF-κB, p65), phosphorylation of mitogen-activated protein kinases (MAPK), reactive oxygen species (ROS) generation, mitochondrial membrane potential and proapoptotic proteins were examined. A-253 cells were pre-treated with DP for 12 h before TNF-α stimulation. 

Results: We observed translocation of NF-κB into the nuclei, prolonged MAPK, excessive ROS generation and strongly decreased mitochondrial membrane potential, and subsequently cytochrome C release and caspase-3 activation. However, pre-treatment with DP significantly inhibited the TNF-α-induced apoptotic factors. 

Conclusions: Our data suggested the inhibitory effect of DP on TNF-α-induced apoptosis in a human salivary gland cell line. This inhibition indicated potential inference of DP in the initial plasma membrane-bound complex of TNF-α and its receptors.

Original languageEnglish
Pages (from-to)313-324
Number of pages12
JournalInflammation Research
Volume62
Issue number3
Early online date25 Dec 2012
DOIs
Publication statusPublished - Mar 2013
Externally publishedYes

Scopus Subject Areas

  • Immunology
  • Pharmacology

User-Defined Keywords

  • A-253
  • Apoptosis
  • Dendrobium officinale
  • Polysaccharides
  • TNF-α

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