Polymorphic microsatellite markers in the long-spined sea urchin Diadema setosum

Mingling Wang; Zhe Li; Monthon Ganmanee; James Y. Xie; Juan Diego Urriago; Jian Wen Qiu

doi:10.1007/s12686-014-0264-y

Polymorphic microsatellite markers in the long-spined sea urchin Diadema setosum

Mingling Wang, Zhe Li, Monthon Ganmanee, James Y. Xie, Juan Diego Urriago, Jian Wen Qiu^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Letter › peer-review

Abstract

We developed twenty polymorphic microsatellite markers for the long-spined sea urchin Diadema setosum by screening microsatellite enriched library, and characterized their polymorphism using two populations of 45 individuals. The number of alleles per locus ranged from 4 to 17, and the observed and expected heterozygosity varied from 0.0714 to 1.000 and 0.6655 to 0.9321, respectively. Twelve loci conformed to the Hardy–Weinberg equilibrium after Bonferroni correction. These polymorphic microsatellite markers will facilitate studies of population genetics and connectivity in D. setosum.

Original language	English
Pages (from-to)	983-985
Number of pages	3
Journal	Conservation Genetics Resources
Volume	6
Issue number	4
DOIs	https://doi.org/10.1007/s12686-014-0264-y
Publication status	Published - Dec 2014

Scopus Subject Areas

Ecology, Evolution, Behavior and Systematics
Genetics

User-Defined Keywords

Diadema
Microsatellite markers
Polymorphism
Sea urchin

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10.1007/s12686-014-0264-y

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title = "Polymorphic microsatellite markers in the long-spined sea urchin Diadema setosum",

abstract = "We developed twenty polymorphic microsatellite markers for the long-spined sea urchin Diadema setosum by screening microsatellite enriched library, and characterized their polymorphism using two populations of 45 individuals. The number of alleles per locus ranged from 4 to 17, and the observed and expected heterozygosity varied from 0.0714 to 1.000 and 0.6655 to 0.9321, respectively. Twelve loci conformed to the Hardy–Weinberg equilibrium after Bonferroni correction. These polymorphic microsatellite markers will facilitate studies of population genetics and connectivity in D. setosum.",

keywords = "Diadema, Microsatellite markers, Polymorphism, Sea urchin",

author = "Mingling Wang and Zhe Li and Monthon Ganmanee and Xie, {James Y.} and Urriago, {Juan Diego} and Qiu, {Jian Wen}",

note = "Funding Information: Acknowledgments This work was supported by Environment and Conservation Fund, Hong Kong (project 2011-08) and Hong Kong Baptist University (project FRG2/13-14/009).",

year = "2014",

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doi = "10.1007/s12686-014-0264-y",

language = "English",

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journal = "Conservation Genetics Resources",

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TY - JOUR

T1 - Polymorphic microsatellite markers in the long-spined sea urchin Diadema setosum

AU - Wang, Mingling

AU - Li, Zhe

AU - Ganmanee, Monthon

AU - Xie, James Y.

AU - Urriago, Juan Diego

AU - Qiu, Jian Wen

N1 - Funding Information: Acknowledgments This work was supported by Environment and Conservation Fund, Hong Kong (project 2011-08) and Hong Kong Baptist University (project FRG2/13-14/009).

PY - 2014/12

Y1 - 2014/12

N2 - We developed twenty polymorphic microsatellite markers for the long-spined sea urchin Diadema setosum by screening microsatellite enriched library, and characterized their polymorphism using two populations of 45 individuals. The number of alleles per locus ranged from 4 to 17, and the observed and expected heterozygosity varied from 0.0714 to 1.000 and 0.6655 to 0.9321, respectively. Twelve loci conformed to the Hardy–Weinberg equilibrium after Bonferroni correction. These polymorphic microsatellite markers will facilitate studies of population genetics and connectivity in D. setosum.

AB - We developed twenty polymorphic microsatellite markers for the long-spined sea urchin Diadema setosum by screening microsatellite enriched library, and characterized their polymorphism using two populations of 45 individuals. The number of alleles per locus ranged from 4 to 17, and the observed and expected heterozygosity varied from 0.0714 to 1.000 and 0.6655 to 0.9321, respectively. Twelve loci conformed to the Hardy–Weinberg equilibrium after Bonferroni correction. These polymorphic microsatellite markers will facilitate studies of population genetics and connectivity in D. setosum.

KW - Diadema

KW - Microsatellite markers

KW - Polymorphism

KW - Sea urchin

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DO - 10.1007/s12686-014-0264-y

M3 - Letter

AN - SCOPUS:84919921262

SN - 1877-7252

VL - 6

SP - 983

EP - 985

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 4

ER -

Polymorphic microsatellite markers in the long-spined sea urchin Diadema setosum

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