TY - JOUR
T1 - Polycyclic aromatic hydrocarbons (PAHs) in different indoor dusts and their potential cytotoxicity based on two human cell lines
AU - Kang, Yuan
AU - Cheung, Kwai Chung
AU - Wong, Ming H.
N1 - Funding Information:
The authors would like to thank Dr. Anna O.W. Leung for editing the manuscript and Dr. Homan Leung for field sampling. Financial support from the Research Grants Council of the University Grants Committee of Hong Kong ( HKBU 260209 ) and Special Equipment Grant ( HKBU09 ) and the Mini-AoE (Area of Excellence, RC/AOE/08-09/01 ) Fund from Hong Kong Baptist University are gratefully acknowledged.
PY - 2010/8
Y1 - 2010/8
N2 - In this study, 55 air-conditioner filter dust samples from six different workplaces including commercial office, secondary school, shopping mall, hospital, electronic factory and manufacturing plant in Hong Kong were collected for analyses of PAH concentration and cytotoxicity. Chemical analyses showed that the total PAHs ranged from 1.17 to 25.5μg/g, with the dust samples from manufacturing plant having the highest concentration. MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrasodium bromide) assay was performed to evaluate the cytotoxicity of organic dust extracts using human hepatocellular liver carcinoma cell line (HepG2) and human skin keratinocyte cell line (KERTr). Each organic dust extract showed marked dose-related response. Dust samples from the manufacturing plant showed the highest cytotoxicity. Curve estimation indicated that power model was fit for explaining the relationship between the total PAH concentration and lethal concentration 50 (LC50). In addition, a significant negative correlation was observed between the total PAH concentration and LC50 both on HepG2 (r=-0.65, p<0.01) and KERTr (r=-0.63, p<0.01) cell lines. Source analyses demonstrated that the PAHs in dust were derived from pyrogenic origins.
AB - In this study, 55 air-conditioner filter dust samples from six different workplaces including commercial office, secondary school, shopping mall, hospital, electronic factory and manufacturing plant in Hong Kong were collected for analyses of PAH concentration and cytotoxicity. Chemical analyses showed that the total PAHs ranged from 1.17 to 25.5μg/g, with the dust samples from manufacturing plant having the highest concentration. MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrasodium bromide) assay was performed to evaluate the cytotoxicity of organic dust extracts using human hepatocellular liver carcinoma cell line (HepG2) and human skin keratinocyte cell line (KERTr). Each organic dust extract showed marked dose-related response. Dust samples from the manufacturing plant showed the highest cytotoxicity. Curve estimation indicated that power model was fit for explaining the relationship between the total PAH concentration and lethal concentration 50 (LC50). In addition, a significant negative correlation was observed between the total PAH concentration and LC50 both on HepG2 (r=-0.65, p<0.01) and KERTr (r=-0.63, p<0.01) cell lines. Source analyses demonstrated that the PAHs in dust were derived from pyrogenic origins.
KW - Air-conditioner filter dust
KW - HepG2 cells
KW - Hong Kong
KW - KERTr cells
KW - MTT assay
UR - http://www.scopus.com/inward/record.url?scp=77953614632&partnerID=8YFLogxK
U2 - 10.1016/j.envint.2010.04.006
DO - 10.1016/j.envint.2010.04.006
M3 - Journal article
AN - SCOPUS:77953614632
SN - 0160-4120
VL - 36
SP - 542
EP - 547
JO - Environment International
JF - Environment International
IS - 6
ER -