Polybrominated diphenyl ethers do not affect metamorphosis but alter the proteome of the invasive slipper limpet Crepidula onyx

Joy Mukherjee, Beverly H.K. Po, Man Ying CHIU*, Rudolf S.S. Wu, Pei Yuan Qian, Vengatesen Thiyagarajan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Man-made polybrominated diphenyl ethers (PBDEs) used as flame retardants in various consumer products may be harmful to marine organisms. Larvae of some marine invertebrates, especially invasive species, can develop resistance to PBDEs through altered protein expression patterns or proteome plasticity. This is the first report of a proteomics approach to study BDE-47 induced molecular changes in the invasive limpet Crepidula onyx. Larvae of C. onyx were cultured for 5days (hatching to metamorphosis) in the presence of BDE-47 (1μgL-1). Using a 2-DE proteomics approach with triple quadrupole and high-resolution TOF-MS, we showed that BDE-47 altered the proteome structure but not the growth or metamorphosis of C. onyx larvae. We found eight significant differentially expressed proteins in response to BDE-47, deemed the protein expression signature, consisting of cytoskeletal, stress tolerance, metabolism and energy production related proteins. Our data suggest C. onyx larvae have adequate proteome plasticity to tolerate BDE-47 toxicity.

Original languageEnglish
Pages (from-to)273-281
Number of pages9
JournalMarine Pollution Bulletin
Volume73
Issue number1
DOIs
Publication statusPublished - 15 Aug 2013

Scopus Subject Areas

  • Oceanography
  • Aquatic Science
  • Pollution

User-Defined Keywords

  • Crepidula onyx
  • Environmental proteomics
  • Larval metamorphosis
  • Polybrominated diphenyl ethers (PBDEs)
  • Protein expression signatures

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