TY - JOUR
T1 - Polybrominated diphenyl ethers do not affect metamorphosis but alter the proteome of the invasive slipper limpet Crepidula onyx
AU - Mukherjee, Joy
AU - Po, Beverly H.K.
AU - Chiu, Jill M.Y.
AU - Wu, Rudolf S.S.
AU - Qian, Pei Yuan
AU - Thiyagarajan, Vengatesen
N1 - Funding Information:
This study was supported by the Area of Excellence Project Grant (AoE/P-04/2004) and General Research Funds (Nos. 778309M and 764711M).
PY - 2013/8/15
Y1 - 2013/8/15
N2 - Man-made polybrominated diphenyl ethers (PBDEs) used as flame retardants in various consumer products may be harmful to marine organisms. Larvae of some marine invertebrates, especially invasive species, can develop resistance to PBDEs through altered protein expression patterns or proteome plasticity. This is the first report of a proteomics approach to study BDE-47 induced molecular changes in the invasive limpet Crepidula onyx. Larvae of C. onyx were cultured for 5days (hatching to metamorphosis) in the presence of BDE-47 (1μgL-1). Using a 2-DE proteomics approach with triple quadrupole and high-resolution TOF-MS, we showed that BDE-47 altered the proteome structure but not the growth or metamorphosis of C. onyx larvae. We found eight significant differentially expressed proteins in response to BDE-47, deemed the protein expression signature, consisting of cytoskeletal, stress tolerance, metabolism and energy production related proteins. Our data suggest C. onyx larvae have adequate proteome plasticity to tolerate BDE-47 toxicity.
AB - Man-made polybrominated diphenyl ethers (PBDEs) used as flame retardants in various consumer products may be harmful to marine organisms. Larvae of some marine invertebrates, especially invasive species, can develop resistance to PBDEs through altered protein expression patterns or proteome plasticity. This is the first report of a proteomics approach to study BDE-47 induced molecular changes in the invasive limpet Crepidula onyx. Larvae of C. onyx were cultured for 5days (hatching to metamorphosis) in the presence of BDE-47 (1μgL-1). Using a 2-DE proteomics approach with triple quadrupole and high-resolution TOF-MS, we showed that BDE-47 altered the proteome structure but not the growth or metamorphosis of C. onyx larvae. We found eight significant differentially expressed proteins in response to BDE-47, deemed the protein expression signature, consisting of cytoskeletal, stress tolerance, metabolism and energy production related proteins. Our data suggest C. onyx larvae have adequate proteome plasticity to tolerate BDE-47 toxicity.
KW - Crepidula onyx
KW - Environmental proteomics
KW - Larval metamorphosis
KW - Polybrominated diphenyl ethers (PBDEs)
KW - Protein expression signatures
UR - http://www.scopus.com/inward/record.url?scp=84881025787&partnerID=8YFLogxK
U2 - 10.1016/j.marpolbul.2013.05.009
DO - 10.1016/j.marpolbul.2013.05.009
M3 - Journal article
C2 - 23743271
AN - SCOPUS:84881025787
SN - 0025-326X
VL - 73
SP - 273
EP - 281
JO - Marine Pollution Bulletin
JF - Marine Pollution Bulletin
IS - 1
ER -
