Poly-l-lysine-based tissue embedding compatible with matrix-assisted laser desorption ionization-mass spectrometry imaging analysis of dry and fragile aristolochia plants

Yu He, Wenjing Guo, Kailong Luo, Qianqian Sun, Zian Lin, Zongwei CAI

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The integrity of tissue is crucial for a high-quality analysis of matrix-assisted laser desorption ionization-mass spectrometry image (MALDI-MSI). Various embedding media utilized in traditional tissue-sectioning techniques are generally not recommended for MALDI-MSI of dry and fragile plant tissues because of the ion suppression effect in MALDI ionization in the low-mass region as well as the undesirable structural deformation during the sample preparation. In this work, a novel poly-L-lysine (PLL)-based tissue embedding method was developed for MALDI-MSI analysis of dry and fragile aristolochia plant (AP) tissues. The practical application in fixation, embedding, cryosectioning, and mounting of the dry and fragile AP tissues demonstrated that the PLL-based embedding technique could provide good rigidity to the plant tissues analysis compared to that without embedding and gelatin embedding. With the assistance of the PLL embedding medium, high spatial resolution molecular ion maps of main compounds, including aristolochic acids I (AAI) and aristolochic acids II (AAII) in AP root tissue, could be achieved by MALDI-MSI with enhanced signal intensities and no obvious background interference. This work provides an alternative approach for embedding the dry and fragile plant tissues comparable with MALDI-MSI analysis.

Original languageEnglish
Pages (from-to)460389
Number of pages1
JournalJournal of Chromatography A
Volume1608
DOIs
Publication statusPublished - 20 Dec 2019

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

User-Defined Keywords

  • Aristolochia plant tissue
  • Aristolochic acids I
  • Aristolochic acids II
  • MALDI-MSI
  • Poly-l-lysine

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