Plant ESCRT protein ALIX coordinates with retromer complex in regulating receptor-mediated sorting of soluble vacuolar proteins

Shuai Hu, Baiying Li, Fan Wu, Dongmei Zhu, Jan Zouhar, Caiji Gao, Tomoo Shimada, Enrique Rojo, Ikuko Hara-Nishimura, Liwen Jiang, Jinbo Shen*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

13 Citations (Scopus)

Abstract

Vacuolar proteins play essential roles in plant physiology and development, but the factors and the machinery regulating their vesicle trafficking through the endomembrane compartments remain largely unknown. We and others have recently identified an evolutionarily conserved plant endosomal sorting complex required for transport (ESCRT)-associated protein apoptosis-linked gene-2 interacting protein X (ALIX), which plays canonical functions in the biogenesis of the multivesicular body/prevacuolar compartment (MVB/PVC) and in the sorting of ubiquitinated membrane proteins. In this study, we elucidate the roles and underlying mechanism of ALIX in regulating vacuolar transport of soluble proteins, beyond its conventional ESCRT function in eukaryotic cells. We show that ALIX colocalizes and physically interacts with the retromer core subunits Vps26 and Vps29 in planta. Moreover, double-mutant analysis reveals the genetic interaction of ALIX with Vps26 and Vps29 for regulating trafficking of soluble vacuolar proteins. Interestingly, depletion of ALIX perturbs membrane recruitment of Vps26 and Vps29 and alters the endosomal localization of vacuolar sorting receptors (VSRs). Taken together, ALIX functions as a unique retromer core subcomplex regulator by orchestrating receptor-mediated vacuolar sorting of soluble proteins.
Original languageEnglish
Article numbere2200492119
Number of pages12
JournalProceedings of the National Academy of Sciences of the United States of America
Volume119
Issue number20
DOIs
Publication statusPublished - 17 May 2022

Scopus Subject Areas

  • General

User-Defined Keywords

  • endosomal recycling
  • ESCRT machiner
  • multivesicular body/prevacuolar compartment (MVB/PVC)
  • retromer complex
  • vacuolar trafficking

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