Phylogenetic Comparison and Splicing Analysis of the U1 snRNP-specific Protein U1C in Eukaryotes

Kai Lu Zhang; Jian Li Zhou; Jing Fang Yang; Yu Zhen Zhao; Debatosh Das; Ge Fei Hao; Caie Wu; Jianhua Zhang; Fu Yuan Zhu; Mo Xian Chen; Shao Ming Zhou

doi:10.3389/fmolb.2021.696319

Phylogenetic Comparison and Splicing Analysis of the U1 snRNP-specific Protein U1C in Eukaryotes

Kai Lu Zhang, Jian Li Zhou, Jing Fang Yang, Yu Zhen Zhao, Debatosh Das, Ge Fei Hao, Caie Wu, Jianhua Zhang, Fu Yuan Zhu, Mo Xian Chen^*, Shao Ming Zhou^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

1 Citation (Scopus)

Abstract

As a pivotal regulator of 5’ splice site recognition, U1 small nuclear ribonucleoprotein (U1 snRNP)-specific protein C (U1C) regulates pre-mRNA splicing by interacting with other components of the U1 snRNP complex. Previous studies have shown that U1 snRNP and its components are linked to a variety of diseases, including cancer. However, the phylogenetic relationships and expression profiles of U1C have not been studied systematically. To this end, we identified a total of 110 animal U1C genes and compared them to homologues from yeast and plants. Bioinformatics analysis shows that the structure and function of U1C proteins is relatively conserved and is found in multiple copies in a few members of the U1C gene family. Furthermore, the expression patterns reveal that U1Cs have potential roles in cancer progression and human development. In summary, our study presents a comprehensive overview of the animal U1C gene family, which can provide fundamental data and potential cues for further research in deciphering the molecular function of this splicing regulator.

Original language	English
Article number	696319
Number of pages	13
Journal	Frontiers in Molecular Biosciences
Volume	8
DOIs	https://doi.org/10.3389/fmolb.2021.696319
Publication status	Published - 9 Sept 2021

Scopus Subject Areas

Biochemistry
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Molecular Biology

User-Defined Keywords

alternative splicing
gene expression
phylogenetics
splice site
U1-snRNP

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3389/fmolb.2021.696319

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@article{1d8cc6184f404df4a286236d9c675667,

title = "Phylogenetic Comparison and Splicing Analysis of the U1 snRNP-specific Protein U1C in Eukaryotes",

abstract = "As a pivotal regulator of 5{\textquoteright} splice site recognition, U1 small nuclear ribonucleoprotein (U1 snRNP)-specific protein C (U1C) regulates pre-mRNA splicing by interacting with other components of the U1 snRNP complex. Previous studies have shown that U1 snRNP and its components are linked to a variety of diseases, including cancer. However, the phylogenetic relationships and expression profiles of U1C have not been studied systematically. To this end, we identified a total of 110 animal U1C genes and compared them to homologues from yeast and plants. Bioinformatics analysis shows that the structure and function of U1C proteins is relatively conserved and is found in multiple copies in a few members of the U1C gene family. Furthermore, the expression patterns reveal that U1Cs have potential roles in cancer progression and human development. In summary, our study presents a comprehensive overview of the animal U1C gene family, which can provide fundamental data and potential cues for further research in deciphering the molecular function of this splicing regulator.",

keywords = "alternative splicing, gene expression, phylogenetics, splice site, U1-snRNP",

author = "Zhang, {Kai Lu} and Zhou, {Jian Li} and Yang, {Jing Fang} and Zhao, {Yu Zhen} and Debatosh Das and Hao, {Ge Fei} and Caie Wu and Jianhua Zhang and Zhu, {Fu Yuan} and Chen, {Mo Xian} and Zhou, {Shao Ming}",

note = "Funding Information: This work was supported by the Natural Science Foundation of Jiangsu Province (SBK2020042924), Jiangsu Agricultural Science and Technology Innovation Found (CX(21)2023), the Science Technology and Innovation Committee of Shenzhen (2021N062-JCYJ20210324115408023), National Natural Science Foundation of China (NSFC31701341, 32001452), NJFU project funding (GXL2018005), and Hong Kong Research Grant Council (AoE/M-05/12, AoE/ M-403/16, GRF14160516, 14177617, 12100318). Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Zhang, Zhou, Yang, Zhao, Das, Hao, Wu, Zhang, Zhu, Chen, Zhou.",

year = "2021",

month = sep,

day = "9",

doi = "10.3389/fmolb.2021.696319",

language = "English",

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journal = "Frontiers in Molecular Biosciences",

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T1 - Phylogenetic Comparison and Splicing Analysis of the U1 snRNP-specific Protein U1C in Eukaryotes

AU - Zhang, Kai Lu

AU - Zhou, Jian Li

AU - Yang, Jing Fang

AU - Zhao, Yu Zhen

AU - Das, Debatosh

AU - Hao, Ge Fei

AU - Wu, Caie

AU - Zhang, Jianhua

AU - Zhu, Fu Yuan

AU - Chen, Mo Xian

AU - Zhou, Shao Ming

N1 - Funding Information: This work was supported by the Natural Science Foundation of Jiangsu Province (SBK2020042924), Jiangsu Agricultural Science and Technology Innovation Found (CX(21)2023), the Science Technology and Innovation Committee of Shenzhen (2021N062-JCYJ20210324115408023), National Natural Science Foundation of China (NSFC31701341, 32001452), NJFU project funding (GXL2018005), and Hong Kong Research Grant Council (AoE/M-05/12, AoE/ M-403/16, GRF14160516, 14177617, 12100318). Publisher Copyright: © Copyright © 2021 Zhang, Zhou, Yang, Zhao, Das, Hao, Wu, Zhang, Zhu, Chen, Zhou.

PY - 2021/9/9

Y1 - 2021/9/9

N2 - As a pivotal regulator of 5’ splice site recognition, U1 small nuclear ribonucleoprotein (U1 snRNP)-specific protein C (U1C) regulates pre-mRNA splicing by interacting with other components of the U1 snRNP complex. Previous studies have shown that U1 snRNP and its components are linked to a variety of diseases, including cancer. However, the phylogenetic relationships and expression profiles of U1C have not been studied systematically. To this end, we identified a total of 110 animal U1C genes and compared them to homologues from yeast and plants. Bioinformatics analysis shows that the structure and function of U1C proteins is relatively conserved and is found in multiple copies in a few members of the U1C gene family. Furthermore, the expression patterns reveal that U1Cs have potential roles in cancer progression and human development. In summary, our study presents a comprehensive overview of the animal U1C gene family, which can provide fundamental data and potential cues for further research in deciphering the molecular function of this splicing regulator.

AB - As a pivotal regulator of 5’ splice site recognition, U1 small nuclear ribonucleoprotein (U1 snRNP)-specific protein C (U1C) regulates pre-mRNA splicing by interacting with other components of the U1 snRNP complex. Previous studies have shown that U1 snRNP and its components are linked to a variety of diseases, including cancer. However, the phylogenetic relationships and expression profiles of U1C have not been studied systematically. To this end, we identified a total of 110 animal U1C genes and compared them to homologues from yeast and plants. Bioinformatics analysis shows that the structure and function of U1C proteins is relatively conserved and is found in multiple copies in a few members of the U1C gene family. Furthermore, the expression patterns reveal that U1Cs have potential roles in cancer progression and human development. In summary, our study presents a comprehensive overview of the animal U1C gene family, which can provide fundamental data and potential cues for further research in deciphering the molecular function of this splicing regulator.

KW - alternative splicing

KW - gene expression

KW - phylogenetics

KW - splice site

KW - U1-snRNP

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U2 - 10.3389/fmolb.2021.696319

DO - 10.3389/fmolb.2021.696319

M3 - Journal article

AN - SCOPUS:85115607829

SN - 2296-889X

VL - 8

JO - Frontiers in Molecular Biosciences

JF - Frontiers in Molecular Biosciences

M1 - 696319

ER -

Phylogenetic Comparison and Splicing Analysis of the U1 snRNP-specific Protein U1C in Eukaryotes

Abstract

Scopus Subject Areas

User-Defined Keywords

UN SDGs

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