A 96-h exposure experiment was conducted to elucidate the toxicity responses of the marine diatom Thalassiosira pseudonana upon exposure to different temperatures and copper (Cu) concentrations. Three Cu treatments (seawater control; 200 μg/L Cu, EC50 for the yield at 25 °C; and 1000 μg/L Cu, EC50 for growth inhibition at 25 °C) were conducted against four temperatures (10 °C, 15 °C, 25 °C and 30 °C). Growth rate and photosynthetic responses showed a significant interacting thermal-chemical effect with strong synergistic responses observed at 30 °C treatments. Expression of heat shock protein (hsp) was positively modulated by increasing temperatures. Hsp 90, hsp90–2 and sit1 (related to silica shell formation) were highly expressed at 30 °C under 1000 μg/L Cu, while the genes encoding light harvesting proteins (3HfcpA and 3HfcpB) and silaffin precursor sil3 were significantly up-regulated at 15 °C under 200 μg/L Cu. Our results indicated an increase Cu toxicity to T. pseudonana under high temperature and Cu dose.
|Number of pages||8|
|Journal||Marine Pollution Bulletin|
|Publication status||Published - 30 Nov 2017|
- Marine diatom
- Thermal stress
- Heat shock protein