Photodynamic therapy induced production of cytokines by latent Epstein Barr virus infected epithelial tumor cells

H. K. Koon; K. W. Lo; M. L. Lung; C. K.C. Chang; Ricky N S WONG; Nai Ki MAK

doi:10.1117/12.710533

Photodynamic therapy induced production of cytokines by latent Epstein Barr virus infected epithelial tumor cells

H. K. Koon^*, K. W. Lo, M. L. Lung, C. K.C. Chang, Ricky N S WONG, Nai Ki MAK

^*Corresponding author for this work

Department of Biology

Research output: Chapter in book/report/conference proceeding › Conference proceeding › peer-review

Abstract

Photodynamic therapy (PDT) is a method to treat cancer or non-cancer diseases by activation of the light-sensitive photosensitizers. Epstein Barr virus (EBV) has been implicated in the development of certain cancers such as nasopharyngeal carcinoma and B cell lymphoma. This study aims to examine the effects of EBV infection on the production of pro-inflammatory cytokines and chemokines in cells after the photosensitizer Zn-BC-AM PDT treatment. Epithelial tumor cell lines HONE-1 and latent EBV-infected HONE-1 (EBV-HONE-1) cells were used in this study. Cells were treated with the photosensitizer Zn-BC-AM for 24 hours before light irradiation. RT-PCR and quantitative ELISA methods were used for the evaluation of mRNA expression and production of cytokines, respectively. Results show that Zn-BC-AM PDT increases the production of IL-1α and IL-1β in EBV-HONE-1. Over a 10-fold increase in the production of H-6 was observed in the culture supernatant of Zn-BC-AM PDTtreated HONE-1 cells. PDT-induced IL-6 production was observed in HONE-1 cells. EBV-HONE-1 has a higher background level of IL-8 production than the HONE-1. The production of IL-8 was suppressed in EBV-HONE-1 cells after Zn-BC-AM PDT. Our results indicate that the response of HONE-1 cells to Zn-BC-AM PDT depends on the presence of latent EBV infection. Since IL-8 is a cytokine with angiogenic activity, Zn-BC-AM PDT may exert an anti-angiogenic effect through the suppression of IL-8 production by the EBV-infected cells.

Original language	English
Title of host publication	Biophotonics and Immune Responses II
DOIs	https://doi.org/10.1117/12.710533
Publication status	Published - 2007
Event	Biophotonics and Immune Responses II - San Jose, CA, United States Duration: 22 Jan 2007 → 23 Jan 2007

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6438
ISSN (Print)	1605-7422

Conference

Conference	Biophotonics and Immune Responses II
Country/Territory	United States
City	San Jose, CA
Period	22/01/07 → 23/01/07

Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

User-Defined Keywords

Cytokines
Epstein-Barr virus
Photodynamic therapy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1117/12.710533

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Koon, H. K., Lo, K. W., Lung, M. L., Chang, C. K. C., WONG, R. N. S., & MAK, N. K. (2007). Photodynamic therapy induced production of cytokines by latent Epstein Barr virus infected epithelial tumor cells. In Biophotonics and Immune Responses II Article 64380M (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6438). https://doi.org/10.1117/12.710533

@inproceedings{b939a350863e4b5c9edc2dffb7bced12,

title = "Photodynamic therapy induced production of cytokines by latent Epstein Barr virus infected epithelial tumor cells",

abstract = "Photodynamic therapy (PDT) is a method to treat cancer or non-cancer diseases by activation of the light-sensitive photosensitizers. Epstein Barr virus (EBV) has been implicated in the development of certain cancers such as nasopharyngeal carcinoma and B cell lymphoma. This study aims to examine the effects of EBV infection on the production of pro-inflammatory cytokines and chemokines in cells after the photosensitizer Zn-BC-AM PDT treatment. Epithelial tumor cell lines HONE-1 and latent EBV-infected HONE-1 (EBV-HONE-1) cells were used in this study. Cells were treated with the photosensitizer Zn-BC-AM for 24 hours before light irradiation. RT-PCR and quantitative ELISA methods were used for the evaluation of mRNA expression and production of cytokines, respectively. Results show that Zn-BC-AM PDT increases the production of IL-1α and IL-1β in EBV-HONE-1. Over a 10-fold increase in the production of H-6 was observed in the culture supernatant of Zn-BC-AM PDTtreated HONE-1 cells. PDT-induced IL-6 production was observed in HONE-1 cells. EBV-HONE-1 has a higher background level of IL-8 production than the HONE-1. The production of IL-8 was suppressed in EBV-HONE-1 cells after Zn-BC-AM PDT. Our results indicate that the response of HONE-1 cells to Zn-BC-AM PDT depends on the presence of latent EBV infection. Since IL-8 is a cytokine with angiogenic activity, Zn-BC-AM PDT may exert an anti-angiogenic effect through the suppression of IL-8 production by the EBV-infected cells.",

keywords = "Cytokines, Epstein-Barr virus, Photodynamic therapy",

author = "Koon, {H. K.} and Lo, {K. W.} and Lung, {M. L.} and Chang, {C. K.C.} and WONG, {Ricky N S} and MAK, {Nai Ki}",

year = "2007",

doi = "10.1117/12.710533",

language = "English",

isbn = "0819465518",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

booktitle = "Biophotonics and Immune Responses II",

}

Koon, HK, Lo, KW, Lung, ML, Chang, CKC, WONG, RNS & MAK, NK 2007, Photodynamic therapy induced production of cytokines by latent Epstein Barr virus infected epithelial tumor cells. in Biophotonics and Immune Responses II., 64380M, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6438, Biophotonics and Immune Responses II, San Jose, CA, United States, 22/01/07. https://doi.org/10.1117/12.710533

Photodynamic therapy induced production of cytokines by latent Epstein Barr virus infected epithelial tumor cells. / Koon, H. K.; Lo, K. W.; Lung, M. L. et al.
Biophotonics and Immune Responses II. 2007. 64380M (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6438).

Research output: Chapter in book/report/conference proceeding › Conference proceeding › peer-review

TY - GEN

T1 - Photodynamic therapy induced production of cytokines by latent Epstein Barr virus infected epithelial tumor cells

AU - Koon, H. K.

AU - Lo, K. W.

AU - Lung, M. L.

AU - Chang, C. K.C.

AU - WONG, Ricky N S

AU - MAK, Nai Ki

PY - 2007

Y1 - 2007

N2 - Photodynamic therapy (PDT) is a method to treat cancer or non-cancer diseases by activation of the light-sensitive photosensitizers. Epstein Barr virus (EBV) has been implicated in the development of certain cancers such as nasopharyngeal carcinoma and B cell lymphoma. This study aims to examine the effects of EBV infection on the production of pro-inflammatory cytokines and chemokines in cells after the photosensitizer Zn-BC-AM PDT treatment. Epithelial tumor cell lines HONE-1 and latent EBV-infected HONE-1 (EBV-HONE-1) cells were used in this study. Cells were treated with the photosensitizer Zn-BC-AM for 24 hours before light irradiation. RT-PCR and quantitative ELISA methods were used for the evaluation of mRNA expression and production of cytokines, respectively. Results show that Zn-BC-AM PDT increases the production of IL-1α and IL-1β in EBV-HONE-1. Over a 10-fold increase in the production of H-6 was observed in the culture supernatant of Zn-BC-AM PDTtreated HONE-1 cells. PDT-induced IL-6 production was observed in HONE-1 cells. EBV-HONE-1 has a higher background level of IL-8 production than the HONE-1. The production of IL-8 was suppressed in EBV-HONE-1 cells after Zn-BC-AM PDT. Our results indicate that the response of HONE-1 cells to Zn-BC-AM PDT depends on the presence of latent EBV infection. Since IL-8 is a cytokine with angiogenic activity, Zn-BC-AM PDT may exert an anti-angiogenic effect through the suppression of IL-8 production by the EBV-infected cells.

AB - Photodynamic therapy (PDT) is a method to treat cancer or non-cancer diseases by activation of the light-sensitive photosensitizers. Epstein Barr virus (EBV) has been implicated in the development of certain cancers such as nasopharyngeal carcinoma and B cell lymphoma. This study aims to examine the effects of EBV infection on the production of pro-inflammatory cytokines and chemokines in cells after the photosensitizer Zn-BC-AM PDT treatment. Epithelial tumor cell lines HONE-1 and latent EBV-infected HONE-1 (EBV-HONE-1) cells were used in this study. Cells were treated with the photosensitizer Zn-BC-AM for 24 hours before light irradiation. RT-PCR and quantitative ELISA methods were used for the evaluation of mRNA expression and production of cytokines, respectively. Results show that Zn-BC-AM PDT increases the production of IL-1α and IL-1β in EBV-HONE-1. Over a 10-fold increase in the production of H-6 was observed in the culture supernatant of Zn-BC-AM PDTtreated HONE-1 cells. PDT-induced IL-6 production was observed in HONE-1 cells. EBV-HONE-1 has a higher background level of IL-8 production than the HONE-1. The production of IL-8 was suppressed in EBV-HONE-1 cells after Zn-BC-AM PDT. Our results indicate that the response of HONE-1 cells to Zn-BC-AM PDT depends on the presence of latent EBV infection. Since IL-8 is a cytokine with angiogenic activity, Zn-BC-AM PDT may exert an anti-angiogenic effect through the suppression of IL-8 production by the EBV-infected cells.

KW - Cytokines

KW - Epstein-Barr virus

KW - Photodynamic therapy

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U2 - 10.1117/12.710533

DO - 10.1117/12.710533

M3 - Conference proceeding

AN - SCOPUS:34548282541

SN - 0819465518

SN - 9780819465511

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Biophotonics and Immune Responses II

T2 - Biophotonics and Immune Responses II

Y2 - 22 January 2007 through 23 January 2007

ER -

Photodynamic therapy induced production of cytokines by latent Epstein Barr virus infected epithelial tumor cells

Abstract

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UN SDGs

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