Photodynamic therapy induced production of cytokines by latent Epstein Barr virus infected epithelial tumor cells

H. K. Koon*, K. W. Lo, M. L. Lung, C. K.C. Chang, Ricky N S WONG, Nai Ki MAK

*Corresponding author for this work

Research output: Chapter in book/report/conference proceedingConference proceedingpeer-review


Photodynamic therapy (PDT) is a method to treat cancer or non-cancer diseases by activation of the light-sensitive photosensitizers. Epstein Barr virus (EBV) has been implicated in the development of certain cancers such as nasopharyngeal carcinoma and B cell lymphoma. This study aims to examine the effects of EBV infection on the production of pro-inflammatory cytokines and chemokines in cells after the photosensitizer Zn-BC-AM PDT treatment. Epithelial tumor cell lines HONE-1 and latent EBV-infected HONE-1 (EBV-HONE-1) cells were used in this study. Cells were treated with the photosensitizer Zn-BC-AM for 24 hours before light irradiation. RT-PCR and quantitative ELISA methods were used for the evaluation of mRNA expression and production of cytokines, respectively. Results show that Zn-BC-AM PDT increases the production of IL-1α and IL-1β in EBV-HONE-1. Over a 10-fold increase in the production of H-6 was observed in the culture supernatant of Zn-BC-AM PDTtreated HONE-1 cells. PDT-induced IL-6 production was observed in HONE-1 cells. EBV-HONE-1 has a higher background level of IL-8 production than the HONE-1. The production of IL-8 was suppressed in EBV-HONE-1 cells after Zn-BC-AM PDT. Our results indicate that the response of HONE-1 cells to Zn-BC-AM PDT depends on the presence of latent EBV infection. Since IL-8 is a cytokine with angiogenic activity, Zn-BC-AM PDT may exert an anti-angiogenic effect through the suppression of IL-8 production by the EBV-infected cells.

Original languageEnglish
Title of host publicationBiophotonics and Immune Responses II
Publication statusPublished - 2007
EventBiophotonics and Immune Responses II - San Jose, CA, United States
Duration: 22 Jan 200723 Jan 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceBiophotonics and Immune Responses II
Country/TerritoryUnited States
CitySan Jose, CA

Scopus Subject Areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

User-Defined Keywords

  • Cytokines
  • Epstein-Barr virus
  • Photodynamic therapy


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