TY - JOUR
T1 - Phosphorus uptake is associated with the rhizosheath formation of mature cluster roots in white lupin under soil drying and phosphorus deficiency
AU - Aslam, Mehtab Muhammad
AU - Karanja, Joseph K.
AU - Yuan, Wei
AU - Zhang, Qian
AU - Zhang, Jianhua
AU - Xu, Weifeng
N1 - We are grateful for the financial support from the National Key Research and Development Program of China ( 2017YFE0118100 ), National Natural Science Foundation of China ( 31761130073, 31422047, 31872169, 31770F123, and 31901428 ). We also sincerely thankful to Dr. Muhammad Waseem, College of Horticulture, South China Agricultural University, Guangzhou, China, for proof reading and language editing of the manuscript.
Publisher Copyright:
© 2021 Elsevier Masson SAS
PY - 2021/9
Y1 - 2021/9
N2 - Phosphorus (P) deficiency largely restricts plant growth and lead to severe yield losses. Therefore, identification of novel root traits to improve P uptake is needed to circumvent yield losses. White lupin (Lupinus albus) is a legume crop that develops cluster roots and has the high phosphorus use efficiency in low P soils. We aimed to investigate the association between cluster roots (CR) rhizosheath formation and P uptake in white lupin. Rhizosheath formation and P concentration were evaluated under four soil treatments. CR increased up to 2.5-fold of overall plant dry weight under SD–P compared to WW + P (control), partly attributable to variations in CR development. Our data showed that SD–P significantly increase rhizosheath weight in white lupin. Among the root segments, MCR showed improved P accumulation in the root which is associated with increased MCR rhizosheath weight. Additionally, a positive correlation was observed between MCR rhizosheath weight and P uptake. Moreover, high sucrose content was recorded in MCR, which may contribute in CR growth under SD–P. Expression analysis of genes related to sucrose accumulation (LaSUC1, LaSUC5, and LaSUC9) and phosphorus uptake (LaSPX3, LaPHO1, and LaPHT1) exhibited peaked expression in MCR under SD-P. This indicate that root sucrose status may facilitate P uptake under P starvation. Together, the ability to enhance P uptake of white lupin is largely associated with MCR rhizosheath under SD–P. Our results showed that gene expression modulation of CR forming plant species, demonstrating that these novel root structures may play crucial role in P acquisition from the soil. Our findings could be implicated for developing P and water efficient crop via CR development in sustainable agriculture.
AB - Phosphorus (P) deficiency largely restricts plant growth and lead to severe yield losses. Therefore, identification of novel root traits to improve P uptake is needed to circumvent yield losses. White lupin (Lupinus albus) is a legume crop that develops cluster roots and has the high phosphorus use efficiency in low P soils. We aimed to investigate the association between cluster roots (CR) rhizosheath formation and P uptake in white lupin. Rhizosheath formation and P concentration were evaluated under four soil treatments. CR increased up to 2.5-fold of overall plant dry weight under SD–P compared to WW + P (control), partly attributable to variations in CR development. Our data showed that SD–P significantly increase rhizosheath weight in white lupin. Among the root segments, MCR showed improved P accumulation in the root which is associated with increased MCR rhizosheath weight. Additionally, a positive correlation was observed between MCR rhizosheath weight and P uptake. Moreover, high sucrose content was recorded in MCR, which may contribute in CR growth under SD–P. Expression analysis of genes related to sucrose accumulation (LaSUC1, LaSUC5, and LaSUC9) and phosphorus uptake (LaSPX3, LaPHO1, and LaPHT1) exhibited peaked expression in MCR under SD-P. This indicate that root sucrose status may facilitate P uptake under P starvation. Together, the ability to enhance P uptake of white lupin is largely associated with MCR rhizosheath under SD–P. Our results showed that gene expression modulation of CR forming plant species, demonstrating that these novel root structures may play crucial role in P acquisition from the soil. Our findings could be implicated for developing P and water efficient crop via CR development in sustainable agriculture.
KW - Cluster roots
KW - P deficient
KW - P uptake
KW - Rhizosheath
KW - Soil drying
KW - White lupin
UR - http://www.scopus.com/inward/record.url?scp=85108410596&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2021.06.022
DO - 10.1016/j.plaphy.2021.06.022
M3 - Journal article
C2 - 34174658
AN - SCOPUS:85108410596
SN - 0981-9428
VL - 166
SP - 531
EP - 539
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
ER -