Pharmacokinetics and metabolism study of veratramine in mice after oral administration using LC-MS/MS

Yue Cong, Jun Li Zhang, Sha Sha Li, Shan Shen, Jiang Ying Wang, Zongwei CAI*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

A simple and sensitive high-performance liquid chromatography coupled with hybrid triple quadrupole–linear ion trap mass spectrometry (Q-trap-MS) method was developed and validated for the determination of veratramine, the major bioactive and neurotoxic component in Veratrum nigrum L. Veratramine and the internal standard (IS) were separated with a Waters Symmetry C18 column and eluted with a gradient mobile phase system containing acetonitrile and 0.1% aqueous formic acid. The analysis was performed by using positive electrospray ionization mode with multiple reaction monitoring (MRM). Transition ions of m/z 410.2 → 295.2 for veratramine and m/z 426.1 → 113.8 for the IS were monitored. The method was validated with a good linearity in the range of 1–1000 ng/mL and lower limit of quantification of 1 ng/mL. The precision (CV) of intra- and inter-day ranged from 3.92 to 7.29%, while the accuracy (bias) intra- and inter-day were between −4.78 and 1.65%. The recovery, stability and matrix effect were within the acceptable ranges. Five metabolites of veratramine, including four hydroxylated and one sulfated metabolites, were tentatively identified using predictive MRM–information dependent acquisition–enhanced product ion mode (predictive MRM-IDA-EPI). The developed method was successfully applied to the pharmacokinetic and metabolic study of veratramine in mice after oral administration of veratramine.

Original languageEnglish
Pages (from-to)1515-1522
Number of pages8
JournalBiomedical Chromatography
Volume30
Issue number9
DOIs
Publication statusPublished - 1 Sep 2016

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

User-Defined Keywords

  • metabolism
  • mouse
  • pharmacokinetics
  • veratramine
  • Veratrum nigrum

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