Pharmacokinetic studies of a Chinese triple herbal drug formula

H. Lv, H. Sun, W. Sun, L. Liu, P. Wang, X. Wang*, H. Cao

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

34 Citations (Scopus)


Yin Chen Hao Tang preparation (YCHTP) is a classic traditional Chinese medicine formula, which is commonly used for clinical treatment of hepatological diseases. In this study, a rapid and validated high-performance liquid chromatography (HPLC) method was developed to simultaneously identify 6,7-dimethylesculetin and geniposide in rat plasma. This assay was performed on a Dikma Diamonsil RP18 column (200 mm×4.6 mm, 5 μm) with acetonitrile-methanol-water (0.1% formic acid) as the mobile phase, showing acceptable linearity, intra- and inter-day precision and accuracy (R.S.D.=5%), and absolute recovery for two analytes (74%); the limits of quantitation were 0.4 and 1.12 μg/ml, and the limits of detection were 0.06 and 0.09 μg/ml for two analytes. The developed method was successfully applied to study the effect of formula compatibility on the pharmacokinetics of 6,7-dimethylesculetin and geniposide in YCHTP when orally administrating an effective human daily dose of YCHTP to rats. We surmise that formula compatibility can significantly influence the pharmacokinetics of YCHTP, and we have elucidated and validated the compatible administration of YCHTP.
Original languageEnglish
Pages (from-to)993 - 1001
Number of pages9
Issue number11
Early online date12 Mar 2008
Publication statusPublished - Nov 2008

User-Defined Keywords

  • Formula compatibility
  • Pharmacokinetics
  • 6,7-Dimethylesculetin
  • Geniposide
  • Yin Chen Hao Tang preparation
  • High-performance liquid chromatography


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