Peptidomics study of anthocyanin-rich juice of elderberry

Hanzhi Wu; Mitch C. Johnson; Chi Hua Lu; Kevin L. Fritsche; Andrew L. Thomas; Yongquan Lai; Zongwei CAI; C. Michael Greenlief

doi:10.1016/j.talanta.2014.08.022

Peptidomics study of anthocyanin-rich juice of elderberry

Hanzhi Wu, Mitch C. Johnson, Chi Hua Lu, Kevin L. Fritsche, Andrew L. Thomas, Yongquan Lai, Zongwei CAI^*, C. Michael Greenlief

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

3 Citations (Scopus)

Abstract

Biologically active peptides play a role in plant signaling and defense. Elderberry juice is known to contain a variety of anthocyanin compounds, a sub-set of polyphenols, which are responsible for the deep purple color of the juice. In this paper, we describe a method utilizing solid phase extraction (SPE) to remove anthocyanins from peptides. Liquid chromatography coupled with tandem mass spectrometry was used to separate and identify the peptides. The results showed that the use of SPE was an effective method to separate peptides from anthocyanins and other background compounds including high polyphenol content in the juice samples. More than 1000 peptides present in elderberry juice were successfully identified.

Original language	English
Pages (from-to)	640-644
Number of pages	5
Journal	Talanta
Volume	131
DOIs	https://doi.org/10.1016/j.talanta.2014.08.022
Publication status	Published - Jan 2015

User-Defined Keywords

Anthocyanin sambucus
Elderberry
Orbitrap-MS/MS
Peptidomics
Solid phase extraction

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10.1016/j.talanta.2014.08.022

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@article{55e5d28e4a634de6a6ecd34187569f1e,

title = "Peptidomics study of anthocyanin-rich juice of elderberry",

abstract = "Biologically active peptides play a role in plant signaling and defense. Elderberry juice is known to contain a variety of anthocyanin compounds, a sub-set of polyphenols, which are responsible for the deep purple color of the juice. In this paper, we describe a method utilizing solid phase extraction (SPE) to remove anthocyanins from peptides. Liquid chromatography coupled with tandem mass spectrometry was used to separate and identify the peptides. The results showed that the use of SPE was an effective method to separate peptides from anthocyanins and other background compounds including high polyphenol content in the juice samples. More than 1000 peptides present in elderberry juice were successfully identified.",

keywords = "Anthocyanin sambucus, Elderberry, Orbitrap-MS/MS, Peptidomics, Solid phase extraction",

author = "Hanzhi Wu and Johnson, {Mitch C.} and Lu, {Chi Hua} and Fritsche, {Kevin L.} and Thomas, {Andrew L.} and Yongquan Lai and Zongwei CAI and Greenlief, {C. Michael}",

note = "Funding Information: The authors would like to thank for the financial supports from National Natural Science Foundation of China ( NSFC21275020 ) and Grant P50AT006273 from the National Center for Complementary and Alternative Medicines (NCCAM) , the Office of Dietary Supplements (ODS) , and the National Cancer Institute (NCI) . The contents in this publication are solely the responsibility of the authors and do not necessarily represent the official views of the NCCAM, ODS, NCI, or the National Institutes of Health. ",

year = "2015",

month = jan,

doi = "10.1016/j.talanta.2014.08.022",

language = "English",

volume = "131",

pages = "640--644",

journal = "Talanta",

issn = "0039-9140",

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T1 - Peptidomics study of anthocyanin-rich juice of elderberry

AU - Wu, Hanzhi

AU - Johnson, Mitch C.

AU - Lu, Chi Hua

AU - Fritsche, Kevin L.

AU - Thomas, Andrew L.

AU - Lai, Yongquan

AU - CAI, Zongwei

AU - Greenlief, C. Michael

N1 - Funding Information: The authors would like to thank for the financial supports from National Natural Science Foundation of China ( NSFC21275020 ) and Grant P50AT006273 from the National Center for Complementary and Alternative Medicines (NCCAM) , the Office of Dietary Supplements (ODS) , and the National Cancer Institute (NCI) . The contents in this publication are solely the responsibility of the authors and do not necessarily represent the official views of the NCCAM, ODS, NCI, or the National Institutes of Health.

PY - 2015/1

Y1 - 2015/1

N2 - Biologically active peptides play a role in plant signaling and defense. Elderberry juice is known to contain a variety of anthocyanin compounds, a sub-set of polyphenols, which are responsible for the deep purple color of the juice. In this paper, we describe a method utilizing solid phase extraction (SPE) to remove anthocyanins from peptides. Liquid chromatography coupled with tandem mass spectrometry was used to separate and identify the peptides. The results showed that the use of SPE was an effective method to separate peptides from anthocyanins and other background compounds including high polyphenol content in the juice samples. More than 1000 peptides present in elderberry juice were successfully identified.

AB - Biologically active peptides play a role in plant signaling and defense. Elderberry juice is known to contain a variety of anthocyanin compounds, a sub-set of polyphenols, which are responsible for the deep purple color of the juice. In this paper, we describe a method utilizing solid phase extraction (SPE) to remove anthocyanins from peptides. Liquid chromatography coupled with tandem mass spectrometry was used to separate and identify the peptides. The results showed that the use of SPE was an effective method to separate peptides from anthocyanins and other background compounds including high polyphenol content in the juice samples. More than 1000 peptides present in elderberry juice were successfully identified.

KW - Anthocyanin sambucus

KW - Elderberry

KW - Orbitrap-MS/MS

KW - Peptidomics

KW - Solid phase extraction

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DO - 10.1016/j.talanta.2014.08.022

M3 - Journal article

C2 - 25281152

AN - SCOPUS:84908257511

SN - 0039-9140

VL - 131

SP - 640

EP - 644

JO - Talanta

JF - Talanta

ER -

Peptidomics study of anthocyanin-rich juice of elderberry

Abstract

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