Peptidomics study of anthocyanin-rich juice of elderberry

Hanzhi Wu, Mitch C. Johnson, Chi Hua Lu, Kevin L. Fritsche, Andrew L. Thomas, Yongquan Lai, Zongwei CAI*, C. Michael Greenlief

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

3 Citations (Scopus)


Biologically active peptides play a role in plant signaling and defense. Elderberry juice is known to contain a variety of anthocyanin compounds, a sub-set of polyphenols, which are responsible for the deep purple color of the juice. In this paper, we describe a method utilizing solid phase extraction (SPE) to remove anthocyanins from peptides. Liquid chromatography coupled with tandem mass spectrometry was used to separate and identify the peptides. The results showed that the use of SPE was an effective method to separate peptides from anthocyanins and other background compounds including high polyphenol content in the juice samples. More than 1000 peptides present in elderberry juice were successfully identified.

Original languageEnglish
Pages (from-to)640-644
Number of pages5
Publication statusPublished - Jan 2015

Scopus Subject Areas

  • Analytical Chemistry

User-Defined Keywords

  • Anthocyanin sambucus
  • Elderberry
  • Orbitrap-MS/MS
  • Peptidomics
  • Solid phase extraction


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