TY - JOUR
T1 - Patchouli oil ameliorates acute colitis
T2 - A targeted metabolite analysis of 2,4,6-trinitrobenzenesulfonic acid-induced rats
AU - Yu, Xiuting
AU - Yang, Guanghua
AU - Jiang, Hua
AU - Lin, Shuhai
AU - Liu, Yuhong
AU - Zhang, Xie
AU - Zeng, Huifang
AU - Su, Ziren
AU - Huang, Song
AU - Shen, Linlin
AU - Zhang, Xiaojun
N1 - Funding Information:
The present study was supported by grants from the National Natural Science Foundation of China (project nos. 81303200, 21377106 and 81173534), Guangdong Natural Science Foundation (project nos. S2013010016627 and S2012010008893), Medical Scientific Research Foundation of Guangdong Province (project no. A2013232), Administration of Traditional Chinese Medicine of Guangdong Province (project no. 20132142), the Special Funds from Central Finance of China in Support of the Development of Local Colleges and University (project no. 276, 2014), the China state ?12th Five year Plan? Scientific and Technological Support Scheme (project no. 2012BAI029B09), Hong Kong, Macao and Taiwan Science & Technology Cooperation Program of China (no. 2014DFH30010) and the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2011). The authors gratefully acknowledge this financial support.
PY - 2017/8
Y1 - 2017/8
N2 - The incidence of inflammatory bowel disease (IBD), characterized by chronic, relapsing intestinal inflammation, has continually increased in recent years. A previous study by our group identified five potential metabolic markers possibly associated with the pathology of 2,4,6‑trinitrobenzenesulfonic acid (TNBS)‑induced IBD in rats. The present study aimed to examine the potential therapeutic effects of the essential oil of Pogostemon cablin (also known as patchouli; PO) on TNBS‑induced rats and investigate the concomitant metabolic changes by targeting the previously identified potential markers. Pogostemon cablin is widely used to treat gastrointestinal diseases, including IBD, in China. The results of the present study showed that PO (270 mg/kg, rectal instillation) significantly alleviated colonic damage and reduced disease activity indicators and colonic myeloperoxidase in TNBS‑induced rats. In addition, a targeted metabolic profiling study identified that four metabolites were elevated in the urine of the animals in the TNBS group, which were significantly inhibited by treatment with PO: Two tryptophan metabolites [4‑(2‑aminophenyl)‑2,4‑dioxobutanoic acid and 4,6‑cihydroxyquinoline] and two gut microbial metabolites (phenylacetylglycine and p‑cresol glucuronide). Taken together, these findings suggested that PO ameliorated the symptoms of TNBS‑induced IBD and reversed the metabolic changes potentially associated with TNBS‑induced IBD in rats.
AB - The incidence of inflammatory bowel disease (IBD), characterized by chronic, relapsing intestinal inflammation, has continually increased in recent years. A previous study by our group identified five potential metabolic markers possibly associated with the pathology of 2,4,6‑trinitrobenzenesulfonic acid (TNBS)‑induced IBD in rats. The present study aimed to examine the potential therapeutic effects of the essential oil of Pogostemon cablin (also known as patchouli; PO) on TNBS‑induced rats and investigate the concomitant metabolic changes by targeting the previously identified potential markers. Pogostemon cablin is widely used to treat gastrointestinal diseases, including IBD, in China. The results of the present study showed that PO (270 mg/kg, rectal instillation) significantly alleviated colonic damage and reduced disease activity indicators and colonic myeloperoxidase in TNBS‑induced rats. In addition, a targeted metabolic profiling study identified that four metabolites were elevated in the urine of the animals in the TNBS group, which were significantly inhibited by treatment with PO: Two tryptophan metabolites [4‑(2‑aminophenyl)‑2,4‑dioxobutanoic acid and 4,6‑cihydroxyquinoline] and two gut microbial metabolites (phenylacetylglycine and p‑cresol glucuronide). Taken together, these findings suggested that PO ameliorated the symptoms of TNBS‑induced IBD and reversed the metabolic changes potentially associated with TNBS‑induced IBD in rats.
KW - 2,4,6-trinitrobenzenesulfonic acid
KW - Colitis
KW - Gut microbial metabolites
KW - Patchouli oil
KW - Targeted metabolomics
KW - Tryptophan catabolites
UR - http://www.scopus.com/inward/record.url?scp=85023747009&partnerID=8YFLogxK
U2 - 10.3892/etm.2017.4577
DO - 10.3892/etm.2017.4577
M3 - Journal article
AN - SCOPUS:85023747009
SN - 1792-0981
VL - 14
SP - 1184
EP - 1192
JO - Experimental and Therapeutic Medicine
JF - Experimental and Therapeutic Medicine
IS - 2
ER -
