Pan-genome wide association study of Glaesserella parasuis highlights genes associated with virulence and biofilm formation

You Zhou, Dike Jiang, Xueping Yao, Yan Luo, Zexiao Yang, Meishen Ren, Ge Zhang, Yuanyuan Yu, Aiping Lu, Yin Wang*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review


Glaesserella parasuis is a gram-negative bacterium that causes fibrotic polyserositis and arthritis in pig, significantly affecting the pig industry. The pan-genome of G. parasuis is open. As the number of genes increases, the core and accessory genomes may show more pronounced differences. The genes associated with virulence and biofilm formation are also still unclear due to the diversity of G. parasuis. Therefore, we have applied a pan-genome-wide association study (Pan-GWAS) to 121 strains G. parasuis. Our analysis revealed that the core genome consists of 1,133 genes associated with the cytoskeleton, virulence, and basic biological processes. The accessory genome is highly variable and is a major cause of genetic diversity in G. parasuis. Furthermore, two biologically important traits (virulence, biofilm formation) of G. parasuis were studied via pan-GWAS to search for genes associated with the traits. A total of 142 genes were associated with strong virulence traits. By affecting metabolic pathways and capturing the host nutrients, these genes are involved in signal pathways and virulence factors, which are beneficial for bacterial survival and biofilm formation. This research lays the foundation for further studies on virulence and biofilm formation and provides potential new drug and vaccine targets against G. parasuis.

Original languageEnglish
Article number1160433
Number of pages22
JournalFrontiers in Microbiology
Publication statusPublished - 17 Apr 2023

Scopus Subject Areas

  • Microbiology
  • Microbiology (medical)

User-Defined Keywords

  • biofilm formation
  • Glaesserella parasuis
  • GWAS
  • pan-genome
  • the accessory genome
  • the core genome
  • virulence


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