Overexpression of miR-328-5p influences cell growth and migration to promote NSCLC progression by targeting LOXL4

Yanzhao Ji, Yanting You, Yifen Wu, Min Wang, Qiuxing He, Xinghong Zhou, Liqian Chen, Xiaomin Sun, Yanyan Liu, Xiuqiong Fu, Hiu Yee Kwan, Qiang Zuo, Ren Luo*, Xiaoshan Zhao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background

Lung cancer is the leading cause of cancer-associated mortality worldwide, and most lung cancers are classified as non-small cell lung cancer (NSCLC). MiR-328 influence the progression of multiple tumors, but the role of miR-328-5p in NSCLC has not been elucidated. The aim of this study was to illuminate the oncogenic role and potential molecular mechanisms of the miR-328-5p and lysyl oxidase like 4 (LOXL4) in NSCLC.

Methods

Expression of miR-328-5p was detected by real-time quantitative polymerase chain reaction (qRT-PCR) in tumor and non-tumor adjacent tissues. After Lentivirus-miR-328-5p was employed to intervene this miRNA in NSCLC cell lines, RT-qPCR was used to detect the expression levels of miR-328-5p. Cell Counting Kit-8 (CCK-8), cell colony formation, flow cytometry, wound healing, Transwell assays were used to determine the malignant phenotypes of NSCLC cells. Nude mice models of subcutaneous tumors were established to observe the effect of miR-328-5p on tumorigenesis. Targeting the 3'UTR of LOXL4 by miR-328-5p was verified by integrated analysis including transcriptome sequencing, dual-luciferase and western-blot assays.

Results

High miR-328-5p level was observed in NSCLC cells from The Cancer Genome Atlas (TCGA) database and tumor tissues collected from NSCLC patients. Overexpressed miR-328-5p promoted NSCLC cell proliferation, survival, and migration, and promoted tumor growth in vivo. Knockdown of miR-328-5p suppressed tumorigenic activities. Transcriptome sequencing analysis revealed that LOXL4 was downregulated by miR-328-5p, which was confirmed by dual-luciferase reporter and western-blot assays.

Conclusions

miR-328-5p showed targeted regulation of LOXL4 to promote cell proliferation and migration in NSCLC.
Original languageEnglish
Article number301
JournalAnnals of translational medicine
Volume10
Issue number6
DOIs
Publication statusPublished - Mar 2022

User-Defined Keywords

  • Non-small cell lung cancer (NSCLC)
  • miR-328-5p
  • lysyl oxidase like 4 (LOXL4)

Fingerprint

Dive into the research topics of 'Overexpression of miR-328-5p influences cell growth and migration to promote NSCLC progression by targeting LOXL4'. Together they form a unique fingerprint.

Cite this