TY - JOUR
T1 - Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies
AU - Li, Xueyan
AU - Fan, Dingding
AU - Zhang, Wei
AU - Liu, Guichun
AU - Zhang, Lu
AU - Zhao, Li
AU - Fang, Xiaodong
AU - Chen, Lei
AU - Dong, Yang
AU - Chen, Yuan
AU - Ding, Yun
AU - Zhao, Ruoping
AU - Feng, Mingji
AU - Zhu, Yabing
AU - Feng, Yue
AU - Jiang, Xuanting
AU - Zhu, Deying
AU - Xiang, Hui
AU - Feng, Xikan
AU - Li, Shuaicheng
AU - Wang, Jun
AU - Zhang, Guojie
AU - Kronforst, Marcus.R.
AU - Wang, Wen
N1 - Funding Information:
This project was funded by grants from the National Natural Science Foundation of China (number 31321002), Chinese Academy of Sciences (XDB13000000) and from Yunnan Provincial Science and Technology Department (number 2013HA004) to W.W. M.R.K. and W.Z. were supported by the National Institutes of Health Grant GM108626- 02.
Publisher copyright:
© 2015 Macmillan Publishers Limited. All rights reserved.
PY - 2015/9/10
Y1 - 2015/9/10
N2 - Butterflies are exceptionally diverse but their potential as an experimental system has been limited by the difficulty of deciphering heterozygous genomes and a lack of genetic manipulation technology. Here we use a hybrid assembly approach to construct high-quality reference genomes for Papilio xuthus (contig and scaffold N50: 492 kb, 3.4 Mb) and Papilio machaon (contig and scaffold N50: 81 kb, 1.15 Mb), highly heterozygous species that differ in host plant affiliations, and adult and larval colour patterns. Integrating comparative genomics and analyses of gene expression yields multiple insights into butterfly evolution, including potential roles of specific genes in recent diversification. To functionally test gene function, we develop an efficient (up to 92.5%) CRISPR/Cas9 gene editing method that yields obvious phenotypes with three genes, Abdominal-B, ebony and frizzled. Our results provide valuable genomic and technological resources for butterflies and unlock their potential as a genetic model system.
AB - Butterflies are exceptionally diverse but their potential as an experimental system has been limited by the difficulty of deciphering heterozygous genomes and a lack of genetic manipulation technology. Here we use a hybrid assembly approach to construct high-quality reference genomes for Papilio xuthus (contig and scaffold N50: 492 kb, 3.4 Mb) and Papilio machaon (contig and scaffold N50: 81 kb, 1.15 Mb), highly heterozygous species that differ in host plant affiliations, and adult and larval colour patterns. Integrating comparative genomics and analyses of gene expression yields multiple insights into butterfly evolution, including potential roles of specific genes in recent diversification. To functionally test gene function, we develop an efficient (up to 92.5%) CRISPR/Cas9 gene editing method that yields obvious phenotypes with three genes, Abdominal-B, ebony and frizzled. Our results provide valuable genomic and technological resources for butterflies and unlock their potential as a genetic model system.
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84941584697&partnerID=MN8TOARS
U2 - 10.1038/ncomms9212
DO - 10.1038/ncomms9212
M3 - Journal article
SN - 2041-1723
JO - Nature Communications
JF - Nature Communications
IS - 6
M1 - 8212
ER -