OsTPP1 regulates seed germination through the crosstalk with abscisic acid in rice

Guanqun Wang, Xiaozheng Li, Nenghui Ye, Mingkun Huang, Lei Feng, Haoxuan Li*, Jianhua ZHANG

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Seed germination is essential for direct seeding in rice. It has been demonstrated that trehalose-6-phosphate phosphatase 1 (OsTPP1) plays roles in improving yield and stress tolerance in rice. In this study, the roles of OsTPP1 on seed germination in rice were investigated. The tpp1 mutant germinated slower than the wild-type (WT), which can be restored by exogenous trehalose. tpp1 seeds showed higher ABA content compared with WT seeds. The tpp1 mutant was hypersensitive to ABA and ABA catabolism inhibitor (Dinicozanole). Furthermore, two ABA catabolism genes were downregulated in the tpp1 mutant which were responsible for increased ABA concentrations, and exogenous trehalose increased transcripts of ABA catabolism genes, suggesting that OsTPP1 and ABA catabolism genes acted in the same signaling pathway. Further analysis showed that a transcription factor of OsGAMYB was an activator of OsTPP1, and expression of OsGAMYB was decreased by both the exogenous and endogenous ABA, subsequently reducing the expression of OsTPP1, which suggested a new signaling pathway required for seed germination in rice. In addition, ABA-responsive genes, especially OsABI5, were invoved in OsTPP1-mediated seed germination. Overall, our study provided new pathways in seed germination that OsTPP1 controlled seed germination through crosstalk with the ABA catabolism pathway.

Original languageEnglish
Pages (from-to)1925-1939
Number of pages15
JournalNew Phytologist
Volume230
Issue number5
DOIs
Publication statusPublished - Jun 2021

Scopus Subject Areas

  • Physiology
  • Plant Science

User-Defined Keywords

  • ABA-responsive genes
  • abscisic acid (ABA)
  • GAMYB
  • OsTPP1
  • rice
  • seed germination
  • trehalose

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