TY - JOUR
T1 - Osteoblast-targeted delivery of miR-33-5p attenuates osteopenia development induced by mechanical unloading in mice
AU - Wang, Han
AU - Hu, Zebing
AU - Shi, Fei
AU - Dong, Jingjing
AU - Dang, Lei
AU - Wang, Yixuan
AU - Sun, Zhongyang
AU - Zhou, Hua
AU - Zhang, Shu
AU - Cao, Xinsheng
AU - Zhang, Ge
N1 - Funding Information:
This work was supported by grants from the National Natural Science Foundation of China (Grant nos. 31570939, 81471815 and 31170889) and the Key Pre-research Project of Manned Spaceflight (grant no. 020106).
PY - 2018/2/7
Y1 - 2018/2/7
N2 - A growing body of evidence has revealed that microRNAs (miRNAs) play crucial roles in regulating osteoblasts and bone metabolism. However, the effects of miRNAs in osteoblast mechanotransduction remain to be defined. In this study, we investigated the regulatory effect of miR-33-5p in osteoblasts and tested its anti-osteopenia effect when delivered by an osteoblast-targeting delivery system in vivo. First, we demonstrated that miR-33-5p could promote the activity and mineralization of osteoblasts without influencing their proliferation in vitro. Then our data showed that supplementing miR-33-5p in osteoblasts by a targeted delivery system partially recovered the osteopenia induced by mechanical unloading at the biochemical, microstructural, and biomechanical levels. In summary, our findings demonstrate that miR-33-5p is a key factor in the occurrence and development of the osteopenia induced by mechanical unloading. In addition, targeted delivery of the mimics of miR-33-5p is a promising new strategy for the treatment of pathological osteopenia.
AB - A growing body of evidence has revealed that microRNAs (miRNAs) play crucial roles in regulating osteoblasts and bone metabolism. However, the effects of miRNAs in osteoblast mechanotransduction remain to be defined. In this study, we investigated the regulatory effect of miR-33-5p in osteoblasts and tested its anti-osteopenia effect when delivered by an osteoblast-targeting delivery system in vivo. First, we demonstrated that miR-33-5p could promote the activity and mineralization of osteoblasts without influencing their proliferation in vitro. Then our data showed that supplementing miR-33-5p in osteoblasts by a targeted delivery system partially recovered the osteopenia induced by mechanical unloading at the biochemical, microstructural, and biomechanical levels. In summary, our findings demonstrate that miR-33-5p is a key factor in the occurrence and development of the osteopenia induced by mechanical unloading. In addition, targeted delivery of the mimics of miR-33-5p is a promising new strategy for the treatment of pathological osteopenia.
UR - http://www.scopus.com/inward/record.url?scp=85041856004&partnerID=8YFLogxK
U2 - 10.1038/s41419-017-0210-5
DO - 10.1038/s41419-017-0210-5
M3 - Journal article
C2 - 29415986
AN - SCOPUS:85041856004
SN - 2041-4889
VL - 9
JO - Cell Death and Disease
JF - Cell Death and Disease
IS - 2
M1 - 170
ER -
