OsProT1 and OsProT3 Function to Mediate Proline- and γ-aminobutyric acid-specific Transport in Yeast and are Differentially Expressed in Rice (Oryza sativa L.)

Jin Hong Lin, Zhi Jun Xu, Jia Shi Peng, Jing Zhao, Guo Bin Zhang, Jun Xie, Zhen Xie Yi, Jianhua ZHANG, Ji Ming Gong, Neng Hui Ye*, Shuan Meng

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Background: Proline (Pro) and γ-aminobutyric acid (GABA) play important roles in plant development and stress tolerance. However, the molecular components responsible for the transport of these molecules in rice remain largely unknown. Results: Here we identified OsProT1 and OsProT3 as functional transporters for Pro and GABA. Transient expression of eGFP-OsProTs in plant protoplasts revealed that both OsProT1 and OsProT3 are localized to the plasma membrane. Ectopic expression in a yeast mutant demonstrated that both OsProT1 and OsProT3 specifically mediate transport of Pro and GABA with affinity for Pro in the low affinity range. qRT-PCR analyses suggested that OsProT1 was preferentially expressed in leaf sheathes during vegetative growth, while OsProT3 exhibited relatively high expression levels in several tissues, including nodes, panicles and roots. Interestingly, both OsProT1 and OsProT3 were induced by cadmium stress in rice shoots. Conclusions: Our results suggested that plasma membrane-localized OsProT1 and OsProT3 efficiently transport Pro and GABA when ectopically expressed in yeast and appear to be involved in various physiological processes, including adaption to cadmium stress in rice plants.

Original languageEnglish
Article number79
JournalRice
Volume12
Issue number1
DOIs
Publication statusPublished - 1 Dec 2019

Scopus Subject Areas

  • Agronomy and Crop Science
  • Soil Science
  • Plant Science

User-Defined Keywords

  • GABA
  • OsProT1
  • OsProT3
  • Proline
  • Stress tolerance
  • Transporter

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