TY - JOUR
T1 - OsMND1 regulates early meiosis and improves the seed set rate in polyploid rice
AU - Xiong, Yuguo
AU - Gan, Lu
AU - Hu, Yaping
AU - Sun, Wenchao
AU - Zhou, Xue
AU - Song, Zhaojian
AU - Zhang, Xianhua
AU - Li, Yang
AU - Yang, Zhifan
AU - Xu, Weifeng
AU - ZHANG, Jianhua
AU - He, Yuchi
AU - Cai, Detian
N1 - Funding Information:
This project was supported by the Chinese National Natural Science Foundation (Grant Nos. 31270356, 31271690, and 31571639), 2017 Hubei Science and Technology Department Innovation Team 2017 (CFA023), 2016 Wuhan Yellow Crane Talents (science) Foundation, and SRF for ROCS, SEM (2015-1098). We thank International Science Editing (http://www.internationalscienceediting.com) for editing this manuscript.
Funding Information:
Acknowledgements This project was supported by the Chinese National Natural Science Foundation (Grant Nos. 31270356, 31271690, and 31571639), 2017 Hubei Science and Technology Department Innovation Team 2017 (CFA023), 2016 Wuhan Yellow Crane Talents (science) Foundation, and SRF for ROCS, SEM (2015-1098). We thank International Science Editing (http://www.internatio nalscienceediting.com) for editing this manuscript.
PY - 2019/3/2
Y1 - 2019/3/2
N2 - The meiotic processes of most polyploid rice (Oryza sativa) are genetically abnormal, leading to low pollen fertility, which results in low seed set rates. Some polyploid meiosis stability (PMeS) lines with high seed set rates have been bred but their meiotic mechanisms remain unknown. In this study, we investigated the function of OsMND1 regulated polyploid rice meiosis. OsMND1 localized in the nucleus, and its expression level in panicles of PMeS line HN2026-4x was higher than in HN2026-2x and the other lines without the PMeS background. OsMND1’s overexpression improved pollen fertility and viability, early normal embryo development and the seed set rate in Balilla-4x. However, OsMND1 RNAi in PMeS line HN2026-4x impeded pollen and embryo development significantly. The results of chromosome behavior analyses indicated that OsMND1 participates in stabilizing meiosis by maintaining the balance of pairing, synapsis and recombination during early meiosis. Many univalent, trivalent, and even multivalent systems appeared in the OsMND1 RNAi line, resulting in the presence of many lagging chromosomes. The outcome indicated that OsMND1 plays a critical role in stabilizing meiosis, improving pollen fertility and reducing early embryo abortions, ultimately increasing the seed set rate. Additionally, OsMND1 affected some key meiosis-related gene expression levels. These results raise interesting issues in polyploid breeding theory and application, which require integrated solutions in the future.
AB - The meiotic processes of most polyploid rice (Oryza sativa) are genetically abnormal, leading to low pollen fertility, which results in low seed set rates. Some polyploid meiosis stability (PMeS) lines with high seed set rates have been bred but their meiotic mechanisms remain unknown. In this study, we investigated the function of OsMND1 regulated polyploid rice meiosis. OsMND1 localized in the nucleus, and its expression level in panicles of PMeS line HN2026-4x was higher than in HN2026-2x and the other lines without the PMeS background. OsMND1’s overexpression improved pollen fertility and viability, early normal embryo development and the seed set rate in Balilla-4x. However, OsMND1 RNAi in PMeS line HN2026-4x impeded pollen and embryo development significantly. The results of chromosome behavior analyses indicated that OsMND1 participates in stabilizing meiosis by maintaining the balance of pairing, synapsis and recombination during early meiosis. Many univalent, trivalent, and even multivalent systems appeared in the OsMND1 RNAi line, resulting in the presence of many lagging chromosomes. The outcome indicated that OsMND1 plays a critical role in stabilizing meiosis, improving pollen fertility and reducing early embryo abortions, ultimately increasing the seed set rate. Additionally, OsMND1 affected some key meiosis-related gene expression levels. These results raise interesting issues in polyploid breeding theory and application, which require integrated solutions in the future.
KW - Meiosis
KW - OsMND1
KW - Pollen fertility
KW - Polyploid rice
KW - Seed set rate
UR - http://www.scopus.com/inward/record.url?scp=85060341633&partnerID=8YFLogxK
U2 - 10.1007/s10725-019-00476-4
DO - 10.1007/s10725-019-00476-4
M3 - Journal article
AN - SCOPUS:85060341633
SN - 0167-6903
VL - 87
SP - 341
EP - 356
JO - Plant Growth Regulation
JF - Plant Growth Regulation
IS - 2
ER -