优化分离与鉴定蓝斑背肛海兔口腔神经节蛋白质组

Translated title of the contribution: Optimized separation and identification of proteome from the buccal ganalion of aplysia (Notarcus leachii cirrosus Stimpson)

陈东仕*, 黄河清, 吴韩志, 蔡宗苇

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

A separation method of proteome from the buccal ganglion of aplysia (Notarcus leachii cirrosus Stimpson, NLCS) with two-dimensional polyacrylamide gel electrophoresis(2D-PAGE) was optimized, gaining about 300 protein spots. Mass spectrograms of peptide mass fingerprint (PMF) of 96 protein spots from the BG were obtained by a combined off-line technique of the matrix-assisted laser desorption ionization mass spectrometry(MALDI-TOF MS) and the emzymolysis in gel. Based on the identification via the databases searches and PMF maps, we found that four of 96 proteins in the gel showed the characteristics with high match scores, which were indicated to be framework proteins tubulin, actin, ribulose-1, 5-bisphosphate carboxylase and oxygenase in neurons cell; meanwhile, a peptide mating pheromone precursor was found. Fifty-six proteins in BG were further classified by a LOG tree software according to the search result of subcellular localization.

Translated title of the contributionOptimized separation and identification of proteome from the buccal ganalion of aplysia (Notarcus leachii cirrosus Stimpson)
Original languageChinese (Simplified)
Pages (from-to)1257-1261
Number of pages5
Journal高等学校化学学报
Volume27
Issue number7
Publication statusPublished - Jul 2006

Scopus Subject Areas

  • Chemistry(all)

User-Defined Keywords

  • 2D-PAGE
  • Aplysia
  • Buccal ganglion
  • Mass spectrometry
  • Proteome

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