TY - JOUR
T1 - Optimization of monoclonal antibody production
T2 - Combined effects of potassium acetate and perfusion in a stirred tank bioreactor
AU - Fong, David W F
AU - Zhang, Yuanxing
AU - Yung, Pingpei
N1 - Funding Information:
This work was supported by a grant from the Industry and Technology Development Council, Hong Kong Government.
PY - 1997
Y1 - 1997
N2 - To increase the yield of monoclonal antibody in a hybridoma culture, it is important to optimize the combination of several factors including cell density, antibody productivity per cell, and the duration of the culture. Potassium acetate enhances the production of antibodies by cells but sometimes depresses cell density. The production of anti-(human B-type red blood cell surface antigen) antibody by Cp9B hybridoma was studied. In batch cultures, potassium acetate inhibited Cp9B cells growth and decreased the maximal cell density but the productivity of antibody per cell was increased. The balance of the two effects resulted in a slight decline of antibody production. In a stirred tank bioreactor, the inhibitory effect of potassium acetate on cell density was overcome by applying the perfusion technique with the attachment of a cell-recycling apparatus to the bioreactor. In such a reactor, potassium acetate at 1 g l-1 did not cause a decrease in the cell density, and the antibody concentration in the culture supernatant was increased from 28 μg ml-1 to 38 μg ml-1. Potassium acetate also suppressed the consumption of glucose and the accumulation of lactate in batch cultures, but the glucose and lactate levels were kept stable by applying the perfusion technique in the stirred tank bioreactor.
AB - To increase the yield of monoclonal antibody in a hybridoma culture, it is important to optimize the combination of several factors including cell density, antibody productivity per cell, and the duration of the culture. Potassium acetate enhances the production of antibodies by cells but sometimes depresses cell density. The production of anti-(human B-type red blood cell surface antigen) antibody by Cp9B hybridoma was studied. In batch cultures, potassium acetate inhibited Cp9B cells growth and decreased the maximal cell density but the productivity of antibody per cell was increased. The balance of the two effects resulted in a slight decline of antibody production. In a stirred tank bioreactor, the inhibitory effect of potassium acetate on cell density was overcome by applying the perfusion technique with the attachment of a cell-recycling apparatus to the bioreactor. In such a reactor, potassium acetate at 1 g l-1 did not cause a decrease in the cell density, and the antibody concentration in the culture supernatant was increased from 28 μg ml-1 to 38 μg ml-1. Potassium acetate also suppressed the consumption of glucose and the accumulation of lactate in batch cultures, but the glucose and lactate levels were kept stable by applying the perfusion technique in the stirred tank bioreactor.
KW - hybridoma
KW - monoclonal antibody
KW - potassium acetate
KW - stirred tank perfusion culture
UR - http://www.scopus.com/inward/record.url?scp=0030906328&partnerID=8YFLogxK
U2 - 10.1023/A:1007914004727
DO - 10.1023/A:1007914004727
M3 - Journal article
AN - SCOPUS:0030906328
SN - 0920-9069
VL - 24
SP - 47
EP - 54
JO - Cytotechnology
JF - Cytotechnology
IS - 1
ER -