Optimization and validation of an HPLC-photodiode array detector method for determination of organic acids in vinegar

Yingying Zong, Jia Lin, Hua Xu, Zhihui Jia, Xiupei Yang*, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

A novel isocratic RP-HPLC method with UV photodiode array detection for the determination of seven organic acids in vinegar samples was developed and optimized. Samples were analyzed on a C18 (150 × 4.6 mm id, 5 μm) analytical column. Methanol-0.010 M sodium dihydrogen phosphate buffer adjusted with H3PO4 to pH 2.80 (2 + 98, v/v), was used as the mobile phase. The column oven temperature was optimized at 23.0° C, the flow rate was 0.80 mL/min, and the detection wavelength was 210 nm. Matrix-matched calibration curves were prepared for all analytes, and the correlation coefficients were greater than 0.99. LOD and LOQ ranged from 0.10 to 10.0 and 0.30 to 30.0 μg/mL, respectively. The results for interday and intraday precision and accuracy fell within the ranges specified. Vinegar samples were quantified by the standard addition method. The main advantages of this method are its selectivity, which is one of the main weaknesses of most methods when determining organic acids in complex matrixes, and its simplicity since little sample preparation is needed. Moreover, it is safe and inexpensive with low organic solvent usage.

Original languageEnglish
Pages (from-to)422-430
Number of pages9
JournalJournal of AOAC International
Volume98
Issue number2
DOIs
Publication statusPublished - 1 Mar 2015

Scopus Subject Areas

  • Analytical Chemistry
  • Food Science
  • Environmental Chemistry
  • Agronomy and Crop Science
  • Pharmacology

Fingerprint

Dive into the research topics of 'Optimization and validation of an HPLC-photodiode array detector method for determination of organic acids in vinegar'. Together they form a unique fingerprint.

Cite this