TY - JOUR
T1 - Optimization and validation of an HPLC-photodiode array detector method for determination of organic acids in vinegar
AU - Zong, Yingying
AU - Lin, Jia
AU - Xu, Hua
AU - Jia, Zhihui
AU - Yang, Xiupei
AU - CHOI, Martin M F
N1 - Financial supports from the National Natural Science Foundation of China (No. 21277109), the Program for Scientific and Technological Innovative Team in Sichuan Provincial Universities (No. 2010008), and the Student Training Programs for Innovation and Entrepreneurship in Sichuan Province (No. 201310638044) are gratefully acknowledged.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - A novel isocratic RP-HPLC method with UV photodiode array detection for the determination of seven organic acids in vinegar samples was developed and optimized. Samples were analyzed on a C18 (150 × 4.6 mm id, 5 μm) analytical column. Methanol-0.010 M sodium dihydrogen phosphate buffer adjusted with H3PO4 to pH 2.80 (2 + 98, v/v), was used as the mobile phase. The column oven temperature was optimized at 23.0° C, the flow rate was 0.80 mL/min, and the detection wavelength was 210 nm. Matrix-matched calibration curves were prepared for all analytes, and the correlation coefficients were greater than 0.99. LOD and LOQ ranged from 0.10 to 10.0 and 0.30 to 30.0 μg/mL, respectively. The results for interday and intraday precision and accuracy fell within the ranges specified. Vinegar samples were quantified by the standard addition method. The main advantages of this method are its selectivity, which is one of the main weaknesses of most methods when determining organic acids in complex matrixes, and its simplicity since little sample preparation is needed. Moreover, it is safe and inexpensive with low organic solvent usage.
AB - A novel isocratic RP-HPLC method with UV photodiode array detection for the determination of seven organic acids in vinegar samples was developed and optimized. Samples were analyzed on a C18 (150 × 4.6 mm id, 5 μm) analytical column. Methanol-0.010 M sodium dihydrogen phosphate buffer adjusted with H3PO4 to pH 2.80 (2 + 98, v/v), was used as the mobile phase. The column oven temperature was optimized at 23.0° C, the flow rate was 0.80 mL/min, and the detection wavelength was 210 nm. Matrix-matched calibration curves were prepared for all analytes, and the correlation coefficients were greater than 0.99. LOD and LOQ ranged from 0.10 to 10.0 and 0.30 to 30.0 μg/mL, respectively. The results for interday and intraday precision and accuracy fell within the ranges specified. Vinegar samples were quantified by the standard addition method. The main advantages of this method are its selectivity, which is one of the main weaknesses of most methods when determining organic acids in complex matrixes, and its simplicity since little sample preparation is needed. Moreover, it is safe and inexpensive with low organic solvent usage.
UR - http://www.scopus.com/inward/record.url?scp=84928811421&partnerID=8YFLogxK
U2 - 10.5740/jaoacint.14-164
DO - 10.5740/jaoacint.14-164
M3 - Journal article
C2 - 25905749
AN - SCOPUS:84928811421
SN - 1060-3271
VL - 98
SP - 422
EP - 430
JO - Journal of AOAC International
JF - Journal of AOAC International
IS - 2
ER -